Human/Mouse CCR4 Antibody Summary
Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284
Accession # P51680
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse CCR4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR4
CCR4 (C-C chemokine receptor 4) is a 42 kDa member of the GPCR #1 family of transmembrane proteins. In rodent, functional CCR4 is expressed on fully differentiated CD4+ Th2 cells, invariant CD4+ NKT cells, and neurons. It is the receptor for CCL17/TARC, CCL22/MDC and CKLF1, and responds to these ligands by initiating chemotaxis. Mouse CCR4 is a 7-transmembrane protein that is 360 amino acids (aa) in length. It contains a 39 aa N-terminal extracellular domain, and a 52 aa C-terminal cyoplasmic tail that shows a poly-Ser motif between aa 343-346. Over aa sequences 1-39, 99-111, 176-205 and 268-284 collectively, mouse CCR4 shares 85% and 93% aa identity with human and rat CCR4, respectively.
Product Datasheets
Citations for Human/Mouse CCR4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation.
Authors: Fang JD, Chou HC, Tung HH
J. Biol. Chem., 2010-12-13;286(7):5667-79.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
PLC3 mediates cortactin interaction with WAVE2 in MCP1-induced actin polymerization and cell migration.
Authors: Jagadeesh Janjanam, Giri Kumar Chandaka, Sivareddy Kotla et al.
The American Society of Cell Biology
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dilution: 1:800
secondary antibody dil: 1: 6000
I use to get few non-specific bands.
