Human/Mouse cIAP-2/HIAP-1 Antibody Summary
Asn2-Ser604
Accession # Q13489
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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cIAP‑2/HIAP‑1 in Human Colon Cancer Tissue. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Mouse cIAP-2/ HIAP-1 Monoclonal Antibody (Catalog # MAB817) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.
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Detection of Human and Mouse cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse cIAP-2/HIAP-1 Monoclonal Antibody (Catalog # MAB817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
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cIAP‑2/HIAP‑1 in Human Colon. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Mouse Anti-Human/Mouse cIAP-2/HIAP-1 Monoclonal Antibody (Catalog # MAB817) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.
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Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse cIAP‑2/HIAP‑1 Monoclonal Antibody (Catalog # MAB817). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-2/HIAP-1
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Human cIAP-2 is a 604 amino acid protein that contains 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
- Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Product Datasheets
Citations for Human/Mouse cIAP-2/HIAP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Inhibitor of apoptosis proteins (IAP) antagonist induces T-cell proliferation after cross-presentation by dendritic cells
Authors: EP Hoefsmit, PT van Royen, D Rao, JA Stunnenber, P Dimitriadi, C Lieftink, B Morris, EA Rozeman, ILM Reijers, R Lacroix, H Shehwana, MA Ligtenberg, RL Beijersber, DS Peeper, CU Blank
Cancer Immunology Research, 2023-04-03;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Close Relationship between cIAP2 and Human ARDS Induced by Severe H7N9 Infection
Authors: C Qin, XY Sai, XF Qian, Y Wu, LF Zou, HM Wang, T Bian, Z Yan
Biomed Res Int, 2019-04-07;2019(0):2121357.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dendritic Cell RIPK1 Maintains Immune Homeostasis by Preventing Inflammation and Autoimmunity
Authors: JA O'Donnell, J Lehman, JE Roderick, D Martinez-M, M Zelic, C Doran, N Hermance, S Lyle, M Pasparakis, KA Fitzgerald, A Marshak-Ro, MA Kelliher
J. Immunol., 2017-12-06;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BIRC3 is a biomarker of mesenchymal habitat of glioblastoma, and a mediator of survival adaptation in hypoxia-driven glioblastoma habitats
Authors: D Wang, AE Berglund, RS Kenchappa, RJ MacAulay, JJ Mulé, AB Etame
Sci Rep, 2017-08-24;7(1):9350.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Effect of hyperthermia on TRAIL-induced apoptotic death in human colon cancer cells: development of a novel strategy for regional therapy.
Authors: Yoo J, Lee YJ
J. Cell. Biochem., 2007-06-01;101(3):619-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors.
Authors: Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S
Int. J. Oncol., 2007-05-01;30(5):1163-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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