Home / CXCL12/SDF-1 / Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody

Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody

Catalog #: BAF310
12 Citations Datasheet / COA / SDS
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BAF310
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Citations (12)
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Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CXCL12/SDF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant human (rh) SDF-1 beta is observed and less than 0.1% cross-reactivity with rhIP‑10 and recombinant mouse CRG‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha (R&D Systems, Catalog # 350-NS)
Lys22-Lys89
Accession # Q6ICW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)

Recombinant Human/Feline CXCL12/SDF-1 beta (Catalog # 351-FS)

Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD)

Human/Mouse CXCL12/SDF-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Mouse CXCL12/SDF‑1 Antibody (Catalog # MAB350)

Standard: Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS)

Standard: Recombinant Mouse CXCL12/SDF-1 alpha Protein (Catalog # 460-SD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

References
  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.
Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

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Citations for Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
    Authors: C Fielding, A García-Gar, C Korn, S Gadomski, Z Fang, JL Reguera, JA Pérez-Simó, B Göttgens, S Méndez-Fer
    Nature Communications, 2022-01-27;13(1):543.
    Species: Mouse
    Sample Types: Bone Marrow Lavage Fluid
    Applications: ELISA Detection
  2. Plasma cell dynamics in the bone marrow niche
    Authors: Z Benet, Z Jing, DR Fooksman
    Cell Reports, 2021-02-09;34(6):108733.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  3. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells
    Authors: A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia
    Immunobiology, 2017-11-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  4. A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
    Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
    PLoS ONE, 2017-04-05;12(4):e0174447.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  5. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  6. Cholinergic Signals from the CNS Regulate G-CSF-Mediated HSC Mobilization from Bone Marrow via a Glucocorticoid Signaling Relay
    Authors: H Pierce, D Zhang, C Magnon, D Lucas, JR Christin, M Huggins, GJ Schwartz, PS Frenette
    Cell Stem Cell, 2017-02-09;0(0):.
    Species: Mouse
    Sample Types: Bone Marrow Fluid
    Applications: ELISA Development (Detection)
  7. CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis.
    Authors: Roy, Ishan, Zimmerman, Noah P, Mackinnon, A Craig, Tsai, Susan, Evans, Douglas, Dwinell, Michael
    PLoS ONE, 2014-03-04;9(3):e90400.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  8. Colonic patch and colonic SILT development are independent and differentially regulated events.
    Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
    Mucosal Immunol, 2012-09-19;6(3):511-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
    Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
    Cancer Res., 2008-03-15;68(6):1889-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
    Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
    J. Cell. Physiol., 2006-05-01;207(2):364-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  11. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
    Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
    Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  12. Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo.
    Authors: Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G
    J. Leukoc. Biol., 2003-07-22;74(5):880-8.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development, Western Blot

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