Human/Mouse Desmocollin-1 Antibody Summary
Arg135-Lys691
Accession # P55849
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Desmocollin‑1 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Desmocollin-1 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
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Detection of Desmocollin‑1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
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Desmocollin‑1 in A549 Human Cell Line. Desmocollin-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Desmocollin‑1 in Mouse Skin. Desmocollin-1 was detected in perfusion fixed frozen sections of mouse skin using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hair root sheath. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmocollin-1
DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin.
DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.
Product Datasheets
Citations for Human/Mouse Desmocollin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality
Sci Rep, 2016-08-26;6(0):32399.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
A spontaneous deletion within the desmoglein 3 extracellular domain of mice results in hypomorphic protein expression, immunodeficiency, and a wasting disease phenotype.
Authors: Kountikov E, Poe J, Maclver N, Rathmell J, Tedder T
Am J Pathol, 2014-12-24;185(3):617-30.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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