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Human/Mouse GLI-3 Antibody

Catalog #: AF3690
45 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3690
AF3690-SP
Detection of Mouse GLI‑3 by Western Blot.
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Product Details
Citations (45)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse GLI‑3 antibody by Western Blot. View Larger

Detection of Mouse GLI‑3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Chromatin Immunoprecipitation (ChIP) Detection of GLI-3-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of GLI‑3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thegli-1promoter was detected by standard PCR.

Immunocytochemistry GLI-3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

GLI‑3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-3

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Long Name
GLI-Kruppel family member GLI3
Entrez Gene IDs
2737 (Human); 14634 (Mouse); 140588 (Rat)
Alternate Names
ACLS; ACLSPAPAPHS; ADD; Bph; GCPS; GLI family zinc finger 3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3; GLI-3; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; Greig cephalopolysyndactyly syndrome; oncogene GLI3; PAP-A; PAPA1; PAPB; PAPBDNA-binding protein; Pdn; PHS; PPDIV; Xt; zinc finger protein GLI3

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Citations for Human/Mouse GLI-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Inferring and perturbing cell fate regulomes in human brain organoids
    Authors: JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein
    Nature, 2022-10-05;0(0):.
    Species: Human
    Sample Types: Organoid, Tissue Homogenates, Whole Cells
    Applications: Bioassay, CUT&Tag, IHC
  2. RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines
    Authors: M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol
    Cancers, 2022-09-19;14(18):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  3. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease
    Authors: S Schmidt, MD Luecken, D Trümbach, S Hembach, KM Niedermeie, N Wenck, K Pflügler, C Stautner, A Böttcher, H Lickert, C Ramirez-Su, R Ahmad, MJ Ziller, JC Fitzgerald, V Ruf, WDJ van de Ber, AJ Jonker, T Gasser, B Winner, J Winkler, DM Vogt Weise, F Giesert, FJ Theis, W Wurst
    Nature Communications, 2022-08-16;13(1):4819.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development
    Authors: J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar
    Cell Reports, 2022-02-01;38(5):110312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  5. HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly
    Authors: D Sharma, AJ Mirando, A Leinroth, JT Long, CM Karner, MJ Hilton
    PloS Genetics, 2021-12-20;17(12):e1009982.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy
    Authors: A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth
    Cancers, 2021-04-15;13(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks
    Authors: KH Elliott, X Chen, J Salomone, P Chaturvedi, PA Schultz, SK Balchand, JD Servetas, A Zuniga, R Zeller, B Gebelein, MT Weirauch, KA Peterson, SA Brugmann
    Elife, 2020-10-02;9(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells
    Authors: JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson
    Science Advances, 2020-09-16;6(38):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  9. AP-1 controls the p11-dependent antidepressant response
    Authors: RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard
    Mol. Psychiatry, 2020-05-21;0(0):.
    Species: C. elegans
    Sample Types: Whole Tissue
    Applications: IHC
  10. TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating
    Authors: H Yan, C Chen, H Chen, H Hong, Y Huang, K Ling, J Hu, Q Wei
    Nat Commun, 2020-05-04;11(1):2196.
    Species: C. elegans
    Sample Types: Whole Tissue
    Applications: IHC
  11. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
    Authors: Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim
    Nat Commun, 2020-04-24;11(1):1994.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. NRF2 negatively regulates primary ciliogenesis and hedgehog signaling
    Authors: P Liu, M Dodson, D Fang, E Chapman, DD Zhang
    PLoS Biol., 2020-02-13;18(2):e3000620.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. The formation of the thumb requires direct modulation of Gli3 transcription by Hoxa13
    Authors: MF Bastida, R Pérez-Góme, A Trofka, J Zhu, A Rada-Igles, R Sheth, HS Stadler, S Mackem, MA Ros
    Proc. Natl. Acad. Sci. U.S.A., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  14. Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration
    Authors: EZM Taroc, A Naik, JM Lin, NB Peterson, DL Keefe, E Genis, G Fuchs, R Balasubram, PE Forni
    J. Neurosci., 2019-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
    Authors: A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon
    Sci Rep, 2019-09-25;9(1):13896.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  16. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019-07-09;28(2):541-553.e4.
    Species: Human, Mouse
    Sample Types: Cell Fractions, Cell Lysates
    Applications: Western Blot
  17. Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules
    Authors: K He, X Ma, T Xu, Y Li, A Hodge, Q Zhang, J Torline, Y Huang, J Zhao, K Ling, J Hu
    Nat Commun, 2018-08-17;9(1):3310.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
    Authors: SR Hong, CL Wang, YS Huang, YC Chang, YC Chang, GV Pusapati, CY Lin, N Hsu, HC Cheng, YC Chiang, WE Huang, NC Shaner, R Rohatgi, T Inoue, YC Lin
    Nat Commun, 2018-04-30;9(1):1732.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts
    Authors: A Struchtrup, A Wiegering, B Stork, U Rüther, C Gerhardt
    Autophagy, 2018-02-21;0(0):1-47.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms
    Authors: T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller
    Cell. Mol. Life Sci., 2018-02-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets
    Authors: LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice
    Front Physiol, 2017-12-19;8(0):1036.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  22. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling
    Authors: VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees
    Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction
    Authors: D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma
    PLoS ONE, 2017-10-31;12(10):e0186879.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development
    Authors: J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017-08-04;15(8):e2002063.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation
    Authors: AM Arensdorf, ME Dillard, JM Menke, MW Frank, CO Rock, SK Ogden
    Cell Rep, 2017-06-06;19(10):2074-2087.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  27. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord
    Authors: H Cai, A Liu
    Dev. Biol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  28. Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9
    Sci Rep, 2016-12-21;6(0):39608.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline
    PLoS Genet., 2016-11-01;12(11):e1006351.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Cholesterol activates the G-protein coupled receptor Smoothened to promote morphogenetic signaling
    Elife, 2016-10-05;5(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
    Proc Natl Acad Sci USA, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number
    Authors: U Emechebe, P Kumar P, JM Rozenberg, B Moore, A Firment, T Mirshahi, AM Moon
    Elife, 2016-04-05;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor.
    Authors: Makino S, Zhulyn O, Mo R, Puviindran V, Zhang X, Murata T, Fukumura R, Ishitsuka Y, Kotaki H, Matsumaru D, Ishii S, Hui C, Gondo Y
    PLoS ONE, 2015-03-11;10(3):e0119455.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  35. Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
    Authors: Chong Y, Mann R, Zhao C, Kato M, Beachy P
    Genes Dev, 2015-02-01;29(3):262-76.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Sufu and Kif7 in limb patterning and development.
    Authors: Zhulyn O, Hui C
    Dev Dyn, 2015-01-24;244(3):468-78.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  37. Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis.
    Authors: Hynes, Ann Mari, Giles, Rachel H, Srivastava, Shalabh, Eley, Lorraine, Whitehead, Jennifer, Danilenko, Marina, Raman, Shreya, Slaats, Gisela G, Colville, John G, Ajzenberg, Henry, Kroes, Hester Y, Thelwall, Peter E, Simmons, Nicholas, Miles, Colin G, Sayer, John A
    Proc Natl Acad Sci U S A, 2014-06-19;111(27):9893-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. A switch from low to high Shh activity regulates establishment of limb progenitors and signaling centers.
    Authors: Zhulyn O, Li D, Deimling S, Vakili N, Mo R, Puviindran V, Chen M, Chuang P, Hopyan S, Hui C
    Dev Cell, 2014-04-10;29(2):241-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
    Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N
    J Clin Invest, 2014-04-01;124(5):2059-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. GATA6 is a crucial regulator of Shh in the limb bud.
    Authors: Kozhemyakina, Elena, Ionescu, Andreia, Lassar, Andrew B
    PLoS Genet, 2014-01-09;10(1):e1004072.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  41. Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis.
    Authors: Renault M, Vandierdonck S, Chapouly C, Yu Y, Qin G, Metras A, Couffinhal T, Losordo D, Yao Q, Reynaud A, Jaspard-Vinassa B, Belloc I, Desgranges C, Gadeau A
    Circ Res, 2013-09-17;113(10):1148-58.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  42. Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
    Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
    FASEB J, 2013-04-16;27(8):2977-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
    Authors: Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A
    Am J Pathol, 2012-09-29;181(6):2126-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  44. Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2).
    Authors: Tanimoto Y, Veistinen L, Alakurtti K
    J. Biol. Chem., 2012-04-30;287(25):21429-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  45. The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
    Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
    Genes Dev., 2010-04-01;24(7):670-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Reviews for Human/Mouse GLI-3 Antibody

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Human/Mouse GLI-3 Antibody
By Anonymous on 05/22/2019
Application: WB Sample Tested: Cell Lysates Species: Mouse
Western Blot Human/Mouse GLI-3 Antibody AF3690
Human/Mouse GLI‑3 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse embryo cell lysate Species: Mouse

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBST
Dilution: 1/1000

Western Blot Human/Mouse GLI‑3 Antibody AF3690