Human/Mouse HSP40/DNAJB1 Antibody

Catalog #: AF4145 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4145
AF4145-SP

Detection of Human/Mouse HSP40/DNAJB1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse HSP40/DNAJB1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse HSP40 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HSP40
Met1-Ile340
Accession # P25685

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Knockout Validated

HSP40/DNAJB1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in HSP40/DNAJB1 knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse HSP40/DNAJB1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HSP40/DNAJB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4145) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HSP40/DNAJB1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human HSP40/DNAJB1 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human HSP40/DNAJB1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HSP40/DNAJB1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse HSP40/DNAJB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4145) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HSP40/DNAJB1 at approximately 40 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP40/DNAJB1

Heat shock protein 40 kDa (HSP40) is the human homologue of the bacterial DnaJ heat shock protein. HSP40, also known as HSPF1 and DnaJ Homolog subfamily B member 1 (DNAJB1), is a 340 amino acid, 40 kDa member of the heat shock protein family. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. HSP40 is a stress inducible chaperone that co-localizes with HSP70 and can bind unfolded proteins and prevent protein denaturation and aggregation. The conserved amino terminal J domain can interact with HSP70 and stimulate its ATPase activity. Human HSP40 shares 95% amino acid identity with mouse and rat HSP40.

Long Name

Heat Shock 40 kDa Protein

Entrez Gene IDs

3337 (Human); 81489 (Mouse); 361384 (Rat)

Alternate Names

DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1