Human/Mouse IL-12/IL-35 p35 APC-conjugated Antibody Summary
Arg23-Ser219
Accession # P29459
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑12/IL‑35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry. (A) Human peripheral blood mononuclear cell (PBMCs) or (B) mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 APC-conjugated Monoclonal Antibody (Catalog # IC2191A) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: IL-12/IL-35 p35
p35 is a 34-36 kDa monomeric member of the IL-6 superfamily of molecules. It is 219 amino acids in length, and possibly contains an N-terminal 22 aa signal sequence. In any event, it does not appear to be individually secreted, and its release from cells is commonly assumed to be as part of a disulfide-linked heterodimer linked to either p40 (forming IL-12) or EBI3 (forming IL-35). Multiple cell types are known to express p35, and include keratinocytes, CD138+ plasma cells, naïve B cells, monocyte-derived dendritic cells, macrophages, CD86lo B7-H1hi CD206+ B7-DC+ tolerogenic dendritic cells, neutrophils, activated monocytes and macrophages, trophoblasts, testicular endothelial cells, and T cells, including CD4+ and CD8+ T helper cells, and CD4+ CD25+ Tregs. The presence of the subunit does not necessarily indicate function, as the p35:p40 heterodimer is associated with promoting inflammatory IFN-gamma production by NK cells, while the p35:EBI3 heterodimer is anti-inflammatory through its inhibition of Th17 cell development. Over aa 23-219, human p35 shares 83% and 57% aa sequence identity with pig and mouse p35, respectively.
Product Datasheets
Citations for Human/Mouse IL-12/IL-35 p35 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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IL-18 is required for the TH1-adaptation of TREG cells and the selective suppression of TH17 responses in acute and chronic infections
Authors: Alvarez, F;Istomine, R;Da Silva Lira Filho, A;Al-Aubodah, TA;Huang, D;Okde, R;Olivier, M;Fritz, JH;Piccirillo, CA;
Mucosal immunology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-4R&alpha signaling by CD8&alpha+ dendritic cells contributes to cerebral malaria by enhancing inflammatory, Th1, and cytotoxic CD8+ T cell responses
Authors: X Wu, F Brombacher, ZC Chroneos, CC Norbury, DC Gowda
The Journal of Biological Chemistry, 2021-03-30;0(0):100615.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulatory T cells and their derived cytokine, interleukin-35, reduce pain in experimental autoimmune encephalomyelitis
Authors: SS Duffy, BA Keating, CJ Perera, JG Lees, RS Tonkin, PGS Makker, P Carrive, O Butovsky, G Moalem-Tay
J. Neurosci., 2019-01-16;0(0):.
Species: Mouse
Sample Types: Whole Cells
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Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
J Virol, 2013-02-06;87(8):4372-83.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.
Authors: van Herwijnen M, Wieten L, van der Zee R, van Kooten P, Wagenaar-Hilbers J, Hoek A, den Braber I, Anderton S, Singh M, Meiring H, van Els C, van Eden W, Broere F
Proc Natl Acad Sci U S A, 2012-08-13;109(35):14134-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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