Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody Summary
Arg23-Ser219
Accession # P29459
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12/IL-35 p35
Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
- Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
- Collison, L.W. et al. (2007) Nature 450:566.
- Bardel, E. et al. (2008) J. Immunol. 181:6898.
- Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Product Datasheets
Citations for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks
Authors: AT Alshareeda, E Rakha, A Alghwainem, B Alrfaei, B Alsowayan, A Albugami, AM Alsubayyil, M Abomraee, NK Mohd Zin
PLoS ONE, 2018-11-20;13(11):e0207593.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modulation of the Tumor Microenvironment by CXCR4 Antagonist-Armed Viral Oncotherapy Enhances the Antitumor Efficacy of Dendritic Cell Vaccines against Neuroblastoma in Syngeneic Mice
Authors: M Komorowski, J Tisonczyk, A Kolakowska, R Drozdz, D Kozbor
Viruses, 2018-08-26;10(9):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Reprogramming antitumor immunity against chemoresistant ovarian cancer by a CXCR4 antagonist-armed viral oncotherapy
Authors: Marcin P Komorowski
Mol Ther Oncolytics, 2016-12-14;3(0):16034.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
Authors: Richter C, Thieme S, Bandola J, Laugsch M, Anastassiadis K, Brenner S
PLoS ONE, 2013-04-23;8(4):e62621.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.
Authors: Pina A, de Araujo E, Felonato M, Loures F, Feriotti C, Bernardino S, Barbuto J, Calich V
Infect Immun, 2013-01-22;81(4):1064-77.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells.
Biochem Biophys Res Commun, 2012-11-12;430(1):364-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
J. Immunol., 2011-05-16;186(12):6661-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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