Human/Mouse IRF6 Antibody

Catalog #: MAB6114
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6114
MAB6114-SP

Detection of Human and Mouse IRF6 by Western Blot.

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All IRF6 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IRF6 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse IRF6 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 503404

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IRF6
Ala176-Gln366
Accession # O14896

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse IRF6 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF6 Monoclonal Antibody (Catalog # MAB6114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF6 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF6

Interferon regulatory factor 6 (IRF6), is a transcription factor that controls the proliferation and differentiation of epidermal and epithelial cells. IRF6 interacts with maspin in normal mammary epithelial cells but is down‑regulated during tumor progression. IRF6 polymorphisms cause Van der Woude and popliteal pterygium syndromes of skin, limb, and craniofacial development that are characterized by cleft lip, cleft palate, or lip pits. IRF6 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within aa 176‑366 of the C‑terminal domain, human IRF6 shares 98% aa sequence identity with mouse and rat IRF6.

Long Name

Interferon Regulatory Factor 8

Entrez Gene IDs

3664 (Human); 54139 (Mouse); 364081 (Rat)

Alternate Names

interferon regulatory factor 6; IRF6; IRF-6; LPS; OFC6; OFC6PPS; PIT; PPS; Van der Woude syndrome; VWS; VWS1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse IRF6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Genetic factors define CPO and CLO subtypes of nonsyndromicorofacial cleft
Authors: L Huang, Z Jia, Y Shi, Q Du, J Shi, Z Wang, Y Mou, Q Wang, B Zhang, Q Wang, S Ma, H Lin, S Duan, B Yin, Y Lin, Y Wang, D Jiang, F Hao, L Zhang, H Wang, S Jiang, H Xu, C Yang, C Li, J Li, B Shi, Z Yang
PLoS Genet., 2019-10-14;15(10):e1008357.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr