Human/Mouse Neurturin Antibody

Catalog #: AF477
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF477
AF477-SP

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All Neurturin Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Neurturin Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Neurturin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Neurturin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Neurturin (Catalog # 1297-NE)
Recombinant Mouse Neurturin (Catalog # 477-MN)

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 387-NE) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # 613-FR) coated at 1 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Neurturin in Mouse Embryo. Neurturin was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse Neurturin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF477) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1 - 4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1 - GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5 - 8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).

References

Lin, L-FH. et al. (1993) Science 260:1130.
Kotzbauer, P.T. et al. (1996) Nature 384:467.
Milbrandt, J. et al. (1998) Neuron 20:245.
Baloh, R.H. et al. (1998) Neuron 21:1291.
Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
Baloh, R.H. et al. (1997) Neuron 18:793.
Jing, S. et al. (1996) Cell 85:1113.
Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
Airaksinen, M. and M. Saarma (2002) Nature Review Neuroscience 3:383.

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Alternate Names

Neurturin; NRTN; NTN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Neurturin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
Mol Ther Methods Clin Dev, 2018-02-23;9(0):172-180.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
Nature, 2014-07-27;514(7520):98-101.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Parasympathetic stimulation improves epithelial organ regeneration.
Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
Nat Commun, 2013-01-01;4(0):1494.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC, Neutralization
Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
Neuropharmacology, 2010-02-11;58(7):1114-21.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr
Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease
Authors: Pia Runeberg-Roos, Elisa Piccinini, Anna-Maija Penttinen, Kert Mätlik, Hanna Heikkinen, Satu Kuure et al.
Neurobiology of Disease
Role of neurturin in spontaneous itch and increased nonpeptidergic intraepidermal fiber density in a mouse model of psoriasis
Authors: Kent Sakai, Kristen M. Sanders, Marina R. Youssef, Kevin M. Yanushefski, Liselotte E. Jensen, Gil Yosipovitch et al.
Pain