Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody

Catalog #: BAM763 Datasheet / COA / SDS

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BAM763

Detection of Orexin A/Hypocretin‑1 in Human Hypothalamus.

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Human/Mouse Orexin A/Hypocretin-1 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detect human and mouse Orexin A/Hypocretin‑1.

Source

Monoclonal Mouse IgG₁ Clone # 97505

Purification

Protein A or G purified from ascites

Immunogen

KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Hypothalamus and perfusion fixed frozen sections of Mouse Hypothalamus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Orexin A/Hypocretin‑1 in Human Hypothalamus. Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Human Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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Detection of Orexin A/Hypocretin‑1 in Mouse Hypothalamus. Orexin A/Hypocretin‑1 was detected in perfusion fixed frozen sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin A/Hypocretin-1

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).

References