Human/Mouse Pax5/BSAP Antibody

Catalog #: MAB3487 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3487
MAB3487-SP

Detection of Human Pax5/BSAP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Pax5/BSAP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Pax5 in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1207C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human Pax5
Thr141-His391
Accession # Q02548

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

3-25 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

Immersion fixed Daudi human Burkitt's lymphoma cell line (Positive) & Jurkat human acute T cell leukemia cell line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Pax5/BSAP by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Pax5/BSAP at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Immunohistochemistry

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Pax5/BSAP in Mouse Embryo. Pax5/BSAP was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western

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Detection of Human Pax5/BSAP by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Pax5/BSAP at approximately 56 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

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Detection of Pax5/BSAP in Daudi human Burkitt's lymphoma cell line (Positive) & Jurkat human acute T cell leukemia cell line (Negative). Pax5/BSAP was detected in immersion fixed Daudi human Burkitt's lymphoma cell line (Positive) & Jurkat human acute T cell leukemia cell line (Negative) using Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to Nucleus. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 42 kDa protein belonging to the paired box transcription factor family. It is a developmental regulator that is important for B-cell lineage commitment and development. Human Pax5 is a 391 amino acid (aa) protein containing the paired DNA-binding domain at the N‑terminal region. Several alternatively spliced isoforms with altered C‑terminal regions and possessing different transactivation properties have been described. All isoforms share the N‑terminal 235 aa with full-length Pax5. Human Pax5 shares 99%, 97% and 94% aa sequence identity with mouse, bovine and canine Pax5, respectively.

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Entrez Gene IDs

5079 (Human); 18507 (Mouse)

Alternate Names

B cell specific activator protein; B-cell-specific transcription factor; BSAP; BSAPB-cell lineage specific activator; paired box 5; paired box gene 5 (B-cell lineage specific activator protein); paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; Pax5; transcription factor PAX 5