Human/Mouse PEA-15 Antibody

Catalog #: AF5588 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5588
AF5588-SP

Detection of Human PEA‑15 by Western Blot.

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Human/Mouse PEA-15 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PEA-15 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PEA-15
Ala2-Ala130
Accession # Q15121

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PEA‑15 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PEA-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PEA-15 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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PEA‑15 in Human Brain. PEA-15 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Mouse PEA-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5588) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEA-15

PEA-15 (Phosphoprotein Enriched in Astrocytes of 15 kDa) is a 15 kDa cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130 amino acids (aa) in length. It contains a death effector domain (aa 3‑81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21 aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36‑58. Full-length human and mouse PEA-15 show 99% aa identity.

Long Name

Phosphoprotein Enriched in Astrocytes 15

Entrez Gene IDs

8682 (Human); 18611 (Mouse); 364052 (Rat)

Alternate Names

HMAT1; HMAT115kD; HUMMAT1H; MAT1H; PEA15; PEA-15; PED; PED15 kDa phosphoprotein enriched in astrocytes; phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in diabetes