Human/Mouse Peroxiredoxin 3 Antibody

Catalog #: AF6610
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6610
AF6610-SP

Detection of Human and Mouse Peroxiredoxin 3 by Western Blot.

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Citations (2)

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Human/Mouse Peroxiredoxin 3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Peroxiredoxin 3 in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse (rm) Peroxiredoxin 2 is observed and less than 1% cross-reactivity with recombinant human (rh) Peroxiredoxin 1, rmPeroxiredoxin 5, and rmPeroxiredoxin 6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Peroxiredoxin 3
Met1-Gln257
Accession # P20108

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Peroxiredoxin 3 by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Peroxiredoxin 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6610) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Peroxiredoxin 3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 3

Peroxiredoxin-3 (Prx-3/III; also AOP-1, MER5 and thioredoxin-dependent peroxidase reductase) is a ubiquitous, 22-28 kDa mitochondrial antioxidant enzyme that belongs to the 2-Cys class of the TSA/ahpC family of peroxiredoxins. Prx-3 is known to act as either a homodimer, or a decamer, and scavenge reactive oxygen species generated by oxidative stress. The mouse Prx-3 precursor molecule is 257 amino acids (aa) in length. It contains a cleavable N-terminal 63 aa mitochondrial targeting sequence, plus a 194 aa mature enzyme that shows a thioredoxin domain between aa 64-222. There are two catalytic cysteines, one at Cys109 and another at Cys230 of the precursor. Prx-3 undergoes a phosphorylation at Thr147 that reduces its activity. One potential splice form shows a deletion of Arg149, Lys150 and Arg185. Full-length mouse Prx-3 is 86% and 95% aa identical to human and rat Prx-3, respectively.

Entrez Gene IDs

10935 (Human); 11757 (Mouse); 64371 (Rat)

Alternate Names

Antioxidant protein 1MGC104387; AOP-1; AOP-1MGC24293; AOP1PRO1748; EC 1.11.1.15; HBC189; MER5; Peroxiredoxin 3; Peroxiredoxin III; peroxiredoxin-3; PRDX3; Protein MER5 homolog; prx-III; SP-22; thioredoxin-dependent peroxide reductase, mitochondrial

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human/Mouse Peroxiredoxin 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Electrophiles modulate glutathione reductase activity via alkylation and upregulation of glutathione biosynthesis
Authors: S Jobbagy, DA Vitturi, SR Salvatore, L Turell, MF Pires, E Kansanen, C Batthyany, JR Lancaster, BA Freeman, FJ Schopfer
Redox Biol, 2018-11-22;21(0):101050.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
NOX2 amplifies acetaldehyde-mediated cardiomyocyte mitochondrial dysfunction in alcoholic cardiomyopathy
Sci Rep, 2016-09-14;6(0):32554.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot