Human/Mouse Phospho-Akt (S473) Pan Specific Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Phospho-Akt (S473) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 nm Calyculin for 30 minutes, 1 µg/mL insulin for 5 minutes, or 100 ng/mL Recombinant Human PDGF-AB (Catalog # 222-AB) for 20 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Akt (S473) Pan Specific Monoclonal Antibody (Catalog # MAB887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Akt (S473) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Phospho-Akt (S473) by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 65 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse Phospho-Akt (S473) Pan Specific Monoclonal Antibody (Catalog # MAB887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt
Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1.
Product Datasheets
Citations for Human/Mouse Phospho-Akt (S473) Pan Specific Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells
Authors: K Kwa?niak, J Czarnik-Kw, K Malysheva, K Pogoda, O Korchynsky, P Rybojad, B Karczmarek, J Tabarkiewi
Cells, 2022-01-23;11(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ACPA decreases non-small cell lung cancer line growth through Akt/PI3K and JNK pathways in vitro
Authors: Ö Boyac?o?lu, E Bilgiç, C Varan, E Bilensoy, E Nemutlu, D Sevim, Ç Kocaefe, P Korkusuz
Cell Death & Disease, 2021-01-11;12(1):56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer
Authors: B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller
Nat. Immunol., 2020-03-09;21(4):442-454.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey
Authors: MT Lane, TJ Herda, AC Fry, MA Cooper, MJ Andre, PM Gallagher
Biol Sport, 2017-01-20;34(2):197-203.
Species: Human
Sample Types: Tissue Homogenates
Applications: Infared Microscopy, Western Blot
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