Human/Mouse/Porcine C‑Reactive Protein/CRP Antibody

Catalog # Availability Size / Price Qty
MAB1707
MAB1707-SP
Detection of Human C‑Reactive Protein/CRP by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Porcine C‑Reactive Protein/CRP Antibody Summary

Species Reactivity
Human, Mouse, Porcine
Specificity
Detects rhCRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with rmCRP and rpCRP but no cross-reactivity with rrCRP.
Source
Monoclonal Mouse IgG2A Clone # 232026
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived CRP
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human C-Reactive Protein/CRP antibody by Western Blot. View Larger

Detection of Human C‑Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Porcine C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB1707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.

Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

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Citation for Human/Mouse/Porcine C‑Reactive Protein/CRP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
    J. Virol., 2012-04-11;86(12):6620-31.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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