Human/Mouse Proprotein Convertase 2/PCSK2 Antibody

Catalog #: AF6018
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6018
AF6018-SP

Detection of Human and Mouse Proprotein Convertase 2/PCSK2 by Western Blot.

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All Proprotein Convertase 2/PCSK2 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Proprotein Convertase 2/PCSK2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs and, human and mouse Proprotein Convertase 2/PCSK2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
Gly110-Asn638
Accession # P16519

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 2/
PCSK2 (Catalog # 6018-SE), see our available Western blot detection antibodies.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Proprotein Convertase 2/PCSK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, beta TC-6 mouse beta cell insulinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Proprotein Convertase 2/PCSK2 in HepG2 Human Cell Line. Proprotein Convertase 2/PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human/Mouse Proprotein Convertase 2/PCSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue).This application has not been tested in mouse samples. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

References

Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Long Name

Proprotein Convertase Subtilisin/Kexin Type 2

Entrez Gene IDs

5126 (Human); 18549 (Mouse); 25121 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.94; KEX2-like endoprotease 2; NEC 2; NEC2; NEC2SPC2; neuroendocrine convertase 2; PC2; PC2NEC-2; PCSK2; Prohormone convertase 2; Proprotein Convertase 2; proprotein convertase subtilisin/kexin type 2; SPC2

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Citation for Human/Mouse Proprotein Convertase 2/PCSK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
Nat. Neurosci., 2018-04-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC