Human/Mouse/Rat Acetyl-CoA
Carboxylase alpha /ACACA Antibody

Catalog #: AF6898 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6898
AF6898-SP

Detection of Human, Mouse, and Rat Acetyl-CoA Carboxylase alpha /ACACA by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Acetyl-CoA
Carboxylase alpha /ACACA Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Acetyl-CoA Carboxylase alpha /ACACA in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Acetyl-CoA Carboxylase alpha /ACACA
Pro1185-Phe1352
Accession # Q13085

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

20 µg/mL

A431 human epithelial carcinoma cells and C2C12 mouse myoblast cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Acetyl-CoA Carboxylase alpha /ACACA by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Acetyl-CoA Carboxylase a/ACACA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6898) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Acetyl-CoA Carboxylase a/ACACA at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human/Mouse/Rat Acetyl‑CoA Carboxylase alpha /ACACA by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cells and C2C12 mouse myoblast cells, loaded at 0.2 mg/mL. A specific band was detected for Acetyl‑CoA Carboxylase alpha /ACACA at approximately 380-400 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human/Mouse/Rat Acetyl‑CoA Carboxylase alpha /ACACA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6898) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66‑440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Acetyl-CoA Carboxylase alpha/ACACA

ACAC-A (Acetyl-CoA carboxylase alpha/1; also ACC-1 and biotin carboxylase) is a 260-265 kDa cytoplasmic, phosphorylated biotinyl-enzyme. It is widely expressed, and found to be concentrated in hepatocytes, adipocytes and lactating mammary epithelium. It is one of two gene products (ACAC-B/beta being the other) that catalyze the formation of malonyl-CoA from acetyl-CoA. The formation of malonyl-CoA by ACAC-A is a rate-limiting step in fatty acid synthesis; malonyl-CoA formed by ACAC-B acts as a regulator of CPT-1 during fatty acidoxidation. Human ACAC-A is 2346 amino acids (aa ) in length. It contains an N-terminal acetylated Met, one ATP-Grasp domain (aa 275-466) with an embedded biotin carboxylation domain (aa 117-618), a biotinyl-binding region (aa 752-818), and a carboxyltransferase domain (aa 1698-2194). There are at least 17 utilized phosphorylation sites, and two acetylated Lys. ACAC-A exists as either a dimer or higher-order oligomer. Multiple splice variants exist. One possesses an alternative start site at Met79, a second utilizes an alternative start site 37 aa upstream of the standard site, and a third (called PIII) shows a 17 aa substitution for aa 1-75. Over aa 1185-1352, human ACAC-A shares 95% aa identity with mouse ACAC-A, and 97% aa identity with both ovine and bovine ACAC-A.

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Entrez Gene IDs

31 (Human); 107476 (Mouse); 60581 (Rat)

Alternate Names

ACACA; ACACacetyl-CoA carboxylase 1; ACC1; ACC1ACC; ACCA; ACC-alpha; AcetylCoA Carboxylase alpha; Acetyl-CoA Carboxylase alpha; acetyl-Coenzyme A carboxylase alpha; EC 6.4.1.2