Human/Mouse/Rat Activin A Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 101 | 295 | 498 | 106 | 308 | 529 |
Standard Deviation | 4.2 | 12.5 | 21.9 | 8.4 | 15.5 | 24.8 |
CV% | 4.2 | 4.2 | 4.4 | 7.9 | 5 | 4.7 |
Recovery
The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 105 | 93-114 |
EDTA Plasma (n=4) | 110 | 95-115 |
Heparin Plasma (n=4) | 107 | 99-113 |
Serum (n=4) | 109 | 100-115 |
Linearity
Scientific Data
Preparation and Storage
Background: Activin A
Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.
Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).
Citations for Human/Mouse/Rat Activin A Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Biomarkers of cellular senescence and risk of death in humans
Authors: St Sauver, JL;Weston, SA;Atkinson, EJ;Mc Gree, ME;Mielke, MM;White, TA;Heeren, AA;Olson, JE;Rocca, WA;Palmer, AK;Cummings, SR;Fielding, RA;Bielinski, SJ;LeBrasseur, NK;
Aging cell
Species: Human
Sample Types: Plasma
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Blocking ActRIIB and restoring appetite reverses cachexia and improves survival in mice with lung cancer
Authors: AL Queiroz, E Dantas, S Ramsamooj, A Murthy, M Ahmed, ERM Zunica, RJ Liang, J Murphy, CD Holman, CJ Bare, G Ghahramani, Z Wu, DE Cohen, JP Kirwan, LC Cantley, CL Axelrod, MD Goncalves
Nature Communications, 2022-08-08;13(1):4633.
Species: Human
Sample Types: Serum
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The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients
Authors: M McAleavy, Q Zhang, PJ Ehmann, J Xu, MF Wipperman, D Ajithdoss, L Pan, M Wakai, R Simonson, A Gadi, A Oyejide, SC Hamon, A Boyapati, LG Morton, T Shavlakadz, CA Kyratsous, DJ Glass
Molecular and Cellular Biology, 2021-11-01;0(0):MCB0046721.
Species: Human
Sample Types: Cell Culture Supernates
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Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors
Authors: KA Cho, DW Choi, YH Kim, J Kim, KH Ryu, SY Woo
Cells, 2021-08-23;10(8):.
Species: Mouse
Sample Types: Serum
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Adipose-derived mesenchymal stem cells preserve cardiac function via ANT-1 in dilated cardiomyopathy hamster model
Authors: D Mori, S Miyagawa, T Kido, H Hata, T Ueno, K Toda, T Kuratani, M Oota, K Kawai, H Kurata, H Nishida, Y Sawa
Regenerative Therapy, 2021-07-09;18(0):182-190.
Species: Human
Sample Types: Cell Culture Supernates
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Role of stromal activin A in human pancreatic cancer and metastasis in mice
Authors: G Mancinelli, C Torres, N Krett, J Bauer, K Castellano, R McKinney, D Dawson, G Guzman, R Hwang, S Grimaldo, P Grippo, B Jung
Scientific Reports, 2021-04-12;11(1):7986.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Plasma
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Carfilzomib Improves Bone Metabolism in Patients with Advanced Relapsed/Refractory Multiple Myeloma: Results of the CarMMa Study
Authors: E Terpos, I Ntanasis-S, E Katodritou, MC Kyrtsonis, V Douka, E Spanoudaki, A Papatheodo, E Eleutherak, N Kanellias, M Gavriatopo, P Makras, E Kastritis, MA Dimopoulos
Cancers, 2021-03-12;13(6):.
Species: Human
Sample Types: Serum
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miR299a-5p promotes renal fibrosis by suppressing the antifibrotic actions of follistatin
Authors: N Mehta, R Li, D Zhang, A Soomro, J He, I Zhang, M MacDonald, B Gao, JC Krepinsky
Scientific Reports, 2021-01-08;11(1):88.
Species: Human
Sample Types: Cell Culture Supernates
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Metallothionein-1G suppresses pancreatic cancer cell stemness by limiting activin A secretion via NF-&kappaB inhibition
Authors: K Li, Z Zhang, Y Mei, Q Yang, S Qiao, C Ni, Y Yao, X Li, M Li, D Wei, W Fu, X Guo, X Huang, H Yang
Theranostics, 2021-01-01;11(7):3196-3212.
Species: Human
Sample Types: Cell Culture Supernates
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Neuroadaptations in the dorsal hippocampus underlie cocaine seeking during prolonged abstinence
Authors: CT Werner, S Mitra, BD Auerbach, ZJ Wang, JA Martin, AF Stewart, PH Gobira, M Iida, C An, MM Cobb, A Caccamise, RJ Salvi, RL Neve, AM Gancarz, DM Dietz
Proc Natl Acad Sci U S A, 2020-10-05;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Growth Hormone Reprograms Macrophages toward an Anti-Inflammatory and Reparative Profile in an MAFB-Dependent Manner
Authors: B Soler Pala, C Nieto, P Fajardo, A González d, N Andrés, Á Dominguez-, P Lucas, A Cuenda, JM Rodríguez-, C Martínez-A, R Villares, ÁL Corbí, M Mellado
J. Immunol., 2020-06-26;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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The senescence-associated secretome as an indicator of age and medical risk
Authors: MJ Schafer, X Zhang, A Kumar, EJ Atkinson, Y Zhu, S Jachim, DL Mazula, AK Brown, M Berning, Z Aversa, B Kotajarvi, CJ Bruce, KL Greason, RM Suri, RP Tracy, SR Cummings, TA White, NK LeBrasseur
JCI Insight, 2020-06-18;5(12):.
Species: Human
Sample Types: Plasma
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Activin A Modulates Inflammation in Acute Pancreatitis and Strongly Predicts Severe Disease Independent of Body Mass Index
Authors: AL Thomas, K Castellano, G Mancinelli, Y Xia, J Bauer, C Yazici, G Fantuzzi, RF Hwang, NL Krett, GI Papachrist, DC Whitcomb, B Jung
Clin Transl Gastroenterol, 2020-05-01;11(5):e00152.
Species: Mouse
Sample Types: Plasma
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Hydroxychloroquine Mitigates the Production of 8-Isoprostane and Improves Vascular Dysfunction: Implications for Treating Preeclampsia
Authors: RA Rahman, P Murthi, H Singh, S Gurungsing, B Leaw, JC Mockler, R Lim, EM Wallace
Int J Mol Sci, 2020-04-03;21(7):.
Species: Human
Sample Types: Placenta
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Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia
Authors: C Pan, N Jaiswal Ag, Y Zulia, S Singh, K Sha, JL Mohler, KH Eng, J Chakkalaka, JJ Krolewski, KL Nastiuk
JCI Insight, 2020-03-26;0(0):.
Species: Mouse
Sample Types: Serum
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Microphthalmia transcription factor expression contributes to bone marrow failure in Fanconi anemia
Authors: A Oppezzo, J Bourseguin, E Renaud, P Pawlikowsk, F Rosselli
J. Clin. Invest., 2020-03-02;0(0):.
Species: Human, Mouse
Sample Types: Cell Culture Supernates
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Serum Activin A Levels and Renal Outcomes After Coronary Angiography
Authors: YL Tsai, RH Chou, YW Lu, CT Liu, PH Huang, SJ Lin
Sci Rep, 2020-02-25;10(1):3365.
Species: Human
Sample Types: Serum
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Increased stiffness of the tumor microenvironment in colon cancer stimulates cancer associated fibroblast-mediated prometastatic activin A signaling
Authors: J Bauer, MAB Emon, JJ Staudacher, AL Thomas, J Zessner-Sp, G Mancinelli, N Krett, MT Saif, B Jung
Sci Rep, 2020-01-09;10(1):50.
Species: Human
Sample Types: Cell Culture Supernates
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Urinary Activin A is a novel biomarker reflecting renal inflammation and tubular damage in ANCA-associated vasculitis
Authors: Y Takei, S Takahashi, M Nakasatomi, T Sakairi, H Ikeuchi, Y Kaneko, K Hiromura, Y Nojima, A Maeshima
PLoS ONE, 2019-10-15;14(10):e0223703.
Species: Human
Sample Types: Serum
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Diagnostic and prognostic values of serum activin-a levels in patients with acute respiratory distress syndrome
Authors: JM Kim, JK Lee, SM Choi, J Lee, YS Park, CH Lee, JJ Yim, CG Yoo, YW Kim, SK Han, SM Lee
BMC Pulm Med, 2019-06-25;19(1):115.
Species: Human
Sample Types: Serum
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Interplay between Follistatin, Activin A, and BMP4 Signaling Regulates Postnatal Thymic Epithelial Progenitor Cell Differentiation during Aging
Authors: A Lepletier, ML Hun, MV Hammett, K Wong, H Naeem, M Hedger, K Loveland, AP Chidgey
Cell Rep, 2019-06-25;27(13):3887-3901.e4.
Species: Mouse
Sample Types: Serum
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Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells
Authors: CN Tsai, CL Tsai, JS Yi, HK Kao, Y Huang, CI Wang, YS Lee, KP Chang
Sci Rep, 2019-03-26;9(1):5197.
Species: Human
Sample Types: Cell Culture Supernates
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Blockade of ALK4/5 signaling suppresses cadmium- and erastin-induced cell death in renal proximal tubular epithelial cells via distinct signaling mechanisms
Authors: K Fujiki, H Inamura, T Sugaya, M Matsuoka
Cell Death Differ., 2019-02-25;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Time dependent degeneration of the nigrostriatal tract in mice with 6-OHDA lesioned medial forebrain bundle and the effect of activin A on L-Dopa induced dyskinesia
Authors: P Rentsch, S Stayte, GP Morris, B Vissel
BMC Neurosci, 2019-02-13;20(1):5.
Species: Mouse
Sample Types: Tissue Homogenates
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Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study
Authors: JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland
EBioMedicine, 2019-01-05;0(0):.
Species: Human
Sample Types: Serum
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NF-?B/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossification
Authors: E Barruet, BM Morales, CJ Cain, AN Ton, KL Wentworth, TV Chan, TA Moody, MC Haks, TH Ottenhoff, J Hellman, MC Nakamura, EC Hsiao
JCI Insight, 2018-11-15;3(22):.
Species: Human
Sample Types: Serum
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Activin subfamily peptides predict chronological age in humans
Authors: LV Barrios-Si, M Parnell, ZB Shinwari, GA Chaudhary, T Xenofontos, A van Bekhov, S McArthur, BT Elliott
Physiol Rep, 2018-09-01;6(17):e13823.
Species: Human
Sample Types: Plasma
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Stroma-derived IL-6, G-CSF and Activin-A mediated dedifferentiation of lung carcinoma cells into cancer stem cells
Authors: CFD Rodrigues, E Serrano, MI Patrício, MM Val, P Albuquerqu, J Fonseca, CMF Gomes, AJ Abrunhosa, A Paiva, L Carvalho, MF Botelho, L Almeida, IM Carreira, MC Alpoim
Sci Rep, 2018-08-01;8(1):11573.
Species: Human
Sample Types: Cell Culture Supernates
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Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells
Authors: M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara
PLoS ONE, 2018-07-26;13(7):e0201239.
Species: Mouse
Sample Types: Cell Culture Supernates
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Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats
Authors: J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang
Mol Med Rep, 2018-01-25;17(4):5074-5080.
Species: Rat
Sample Types: Serum
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ACVR2B/Fc counteracts chemotherapy-induced loss of muscle and bone mass
Authors: R Barreto, Y Kitase, T Matsumoto, F Pin, KC Colston, KE Couch, TM O'Connell, ME Couch, LF Bonewald, A Bonetto
Sci Rep, 2017-10-31;7(1):14470.
Species: Mouse
Sample Types: Plasma
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Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
Authors: Jonas J Staudacher, Cemal Yazici, Timothy Carroll, Jessica Bauer, Jingbo Pang, Nancy Krett, Yinglin Xia, Annette Wilson, Georgios Papachris, Andrea Dirmeier, Claudia Kunst, David C Whitcomb, Giamila Fantuzzi, Barbara Jung
Scientific Reports, 2017-10-06;0(0):2045-2322.
Species: Human, Mouse
Sample Types: Serum
Applications: ELISA Capture -
Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
Sci Rep, 2017-08-15;7(1):8225.
Species: Human
Sample Types: Whole Cells
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Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults
Authors: AT Amaraseker, B Assadi-Kha, S Liu, M Black, G Dymmott, NM Rogers, AL Sverdlov, JD Horowitz, DTM Ngo
PLoS ONE, 2017-05-10;12(5):e0174435.
Species: Human
Sample Types: Plasma
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Activin A more prominently regulates muscle mass in primates than does GDF8
Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
Nat Commun, 2017-04-28;8(0):15153.
Species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rat
Sample Types: Serum
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Activin A is increased in the nucleus accumbens following a cocaine binge
Authors: ZJ Wang, JA Martin, AM Gancarz, DN Adank, FJ Sim, DM Dietz
Sci Rep, 2017-03-08;7(0):43658.
Species: Rat
Sample Types: Tissue Homogenates
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Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis
Authors: A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton
Stem Cell Res Ther, 2017-02-07;8(1):22.
Species: Ovine
Sample Types: Plasma
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Activin A Inhibits MPTP and LPS-Induced Increases in Inflammatory Cell Populations and Loss of Dopamine Neurons in the Mouse Midbrain In Vivo
Authors: S Stayte, P Rentsch, AR Tr”scher, M Bamberger, KM Li, B Vissel
PLoS ONE, 2017-01-25;12(1):e0167211.
Species: Mouse
Sample Types: Tissue Homogenates
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A Key Role for Inhibins in Dendritic Cell Maturation and Function
PLoS ONE, 2016-12-09;11(12):e0167813.
Species: Mouse
Sample Types: Cell Culture Supernates
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Involvement of Infiltrating Macrophage-derived Activin A in the Progression of Renal Damage in MRL-lpr Mice
Authors: Yoshihisa Nojima
Am. J. Physiol. Renal Physiol., 2016-11-23;0(0):ajprenal.0019.
Species: Mouse
Sample Types: Urine
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Diet-induced weight loss is sufficient to reduce senescent cell number in white adipose tissue of weight-cycled mice
Authors: Edward O List
Nutr Healthy Aging, 2016-10-27;4(1):95-99.
Species: Mouse
Sample Types: Plasma
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Predicting first trimester pregnancy outcome: derivation of a multiple marker test
Authors: S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart
Fertil. Steril., 2016-10-26;106(7):1725-1732.e3.
Species: Human
Sample Types: Serum
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Trace element and cytokine concentrations in patients with Fibrodysplasia Ossificans Progressiva (FOP): A case control study
Authors: Laura Hildebrand
J Trace Elem Med Biol, 2016-10-04;39(0):186-192.
Species: Human
Sample Types: Serum
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Late acute graft versus host disease: a prospective analysis of clinical outcomes and circulating angiogenic factors
Blood, 2016-09-13;0(0):.
Species: Human
Sample Types: Plasma
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Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes
Authors: Tonia L Vincent
Arthritis & rheumatology (Hoboken, N.J.), 2016-09-01;68(9):2129-40.
Species: Human
Sample Types: Plasma
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Activin A programs the differentiation of human TFH cells
Nat Immunol, 2016-07-04;17(8):976-84.
Species: Human
Sample Types: Cell Culture Supernates
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Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
PLoS ONE, 2015-02-23;10(2):e0118097.
Species: Human
Sample Types: Cell Culture Supernates
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The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway.
Authors: Wilson C, Ottewell P, Coleman R, Holen I
BMC Cancer, 2015-02-14;15(0):55.
Species: Human
Sample Types: Cell Culture Supernates
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Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring.
Authors: Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A
PLoS ONE, 2014-04-24;9(4):e92175.
Species: Human
Sample Types: Serum
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Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
Authors: Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X
Cell Commun Signal, 2014-03-17;12(0):18.
Species: Rat
Sample Types: Cell Culture Supernates
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PMA induces SnoN proteolysis and CD61 expression through an autocrine mechanism.
Authors: Li C, Peart N, Xuan Z, Lewis D, Xia Y, Jin J
Cell Signal, 2014-03-15;26(7):1369-78.
Species: Human
Sample Types: Cell Culture Supernates
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Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes.
Authors: Chen W, Greulich S, van der Meer R, Rijzewijk L, Lamb H, de Roos A, Smit J, Romijn J, Ruige J, Lammertsma A, Lubberink M, Diamant M, Ouwens D
Cardiovasc Diabetol, 2013-10-17;12(1):150.
Species: Human
Sample Types: Plasma
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An essential role for insulin and IGF1 receptors in regulating sertoli cell proliferation, testis size, and FSH action in mice.
Authors: Pitetti J, Calvel P, Zimmermann C, Conne B, Papaioannou M, Aubry F, Cederroth C, Urner F, Fumel B, Crausaz M, Docquier M, Herrera P, Pralong F, Germond M, Guillou F, Jegou B, Nef S
Mol Endocrinol, 2013-03-21;27(5):814-27.
Species: Mouse
Sample Types: Serum
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Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
J. Bone Miner. Res., 2011-08-01;26(8):1891-903.
Species: Human
Sample Types: Serum
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Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
Blood, 2011-03-09;117(19):5092-101.
Species: Mouse
Sample Types: Cell Culture Supernates
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