Human/Mouse/Rat ARK5/NUAK1 Antibody

Catalog #: MAB6978 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6978
MAB6978-SP

Detection of Human, Mouse, and Rat ARK5/NUAK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ARK5/NUAK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human ARK5/NUAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SNARK is observed.

Source

Monoclonal Mouse IgG₁ Clone # 715901

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ARK5/NUAK1
Lys311-Met462
Accession # O60285

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat ARK5/NUAK1 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ARK5/NUAK1 Monoclonal Antibody (Catalog # MAB6978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ARK5/NUAK1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARK5/NUAK1

AMPK-related kinase 5 (ARK5), also known as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short isoform of the human protein is prematurely tuncated within this domain. Within aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5, respectively.

Long Name

AMPK-related Protein Kinase 5

Entrez Gene IDs

9891 (Human); 77976 (Mouse); 299694 (Rat)

Alternate Names

AMPK-related protein kinase 5; ARK5; ARK5AMP-activated protein kinase family member 5; EC 2.7.11; EC 2.7.11.1; KIAA0537NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; NUAK1