Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody Summary
Asn99-Thr193
Accession # Q9H1Y0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of ATG5 antibody in HeLa Human Cell Line antibody by Flow Cytometry. Detection of ATG5 antibody in HeLa Human Cell Line antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/ATG5_IC5294R_Flow_Cytometry_20950.jpg)
Detection of ATG5 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC5294R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ATG5
ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody and earn rewards!
Have you used Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image