Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody

Catalog #: IC5294R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC5294R-100

Detection of ATG5 in HeLa Human Cell Line by Flow Cytometry.

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Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ATG5 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 603813

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ATG5
Asn99-Thr193
Accession # Q9H1Y0

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ATG5 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of ATG5 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat ATG5 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC5294R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.

Long Name

ATG5 Autophagy Related 5 Homolog

Entrez Gene IDs

9474 (Human); 11793 (Mouse)

Alternate Names

APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 autophagy 5-like (S. cerevisiae); APG5; APG5L; APG5-LIKE; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5 Autophagy Related 5 Homolog; ATG5; Autophagy protein 5; Autophagy Related 5; HAPG5; SCAR25