Home / ATG5 / Human/Mouse/Rat ATG5 Antibody

Human/Mouse/Rat ATG5 Antibody

Catalog #: MAB5294
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5294
MAB5294-SP
Detection of Human, Mouse, and Rat ATG5 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ATG5 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ATG5 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 603813
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ATG5
Asn99-Thr193
Accession # Q9H1Y0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
25 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded human small intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat ATG5 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat ATG5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human and Rat ATG5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Rat ATG5 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG5 at approximately 51 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human/Mouse/Rat ATG5 Monoclonal Antibody (Catalog # MAB5294). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5.

Long Name
ATG5 Autophagy Related 5 Homolog
Entrez Gene IDs
9474 (Human); 11793 (Mouse)
Alternate Names
APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 autophagy 5-like (S. cerevisiae); APG5; APG5L; APG5-LIKE; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5 Autophagy Related 5 Homolog; ATG5; Autophagy protein 5; Autophagy Related 5; HAPG5; SCAR25

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Citation for Human/Mouse/Rat ATG5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory
    Authors: P González-R, M Cheray, J Füllgrabe, M Salli, P Engskog-Vl, L Keane, V Cunha, A Lupa, W Li, Q Ma, K Dreij, MG Rosenfeld, B Joseph
    Autophagy, 2020-09-14;0(0):1-19.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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