Human/Mouse/Rat Calretinin Antibody

Catalog #: AF5065
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5065
AF5065-SP

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Calretinin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Calretinin
Ala2-Ser177
Accession # AAH15484

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Calretinin in Human Brain. Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/ Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human/Mouse/Rat Calretinin by Western Blot. Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Calretinin at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calretinin

Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca²⁺-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca²⁺-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.

Entrez Gene IDs

794 (Human); 12308 (Mouse); 117059 (Rat)

Alternate Names

29 kDa calbindin; CAB29; CAL2; CALB2; calbindin 2; calbindin 2, (29kD, calretinin); calbindin 2, 29kDa (calretinin); calbindin D29K; Calretinin; CR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Calretinin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA release
Authors: CE Geisler, S Ghimire, C Hepler, KE Miller, SM Bruggink, KP Kentch, MR Higgins, CT Banek, J Yoshino, S Klein, BJ Renquist
Cell Reports, 2021-06-29;35(13):109298.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Is the eye a window to the brain in Sanfilippo syndrome?
Authors: H Beard, G Chidlow, D Neumann, N Nazri, M Douglass, PJ Trim, MF Snel, RJ Casson, KM Hemsley
Acta Neuropathol Commun, 2020-11-17;8(1):194.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice
Authors: MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa
PLoS ONE, 2019-08-20;14(8):e0221440.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Estrogen treatment reverses prematurity-induced disruption in cortical interneuron population
Authors: S Panda, P Dohare, S Jain, N Parikh, P Singla, R Mehdizadeh, DW Klebe, GM Kleinman, B Cheng, P Ballabh
J. Neurosci., 2018-07-23;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury
Authors: RW Nickells, HM Schmitt, ME Maes, CL Schlamp
Invest. Ophthalmol. Vis. Sci., 2017-12-01;58(14):6091-6104.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
The nucleus reuniens: a key node in the neurocircuitry of stress and depression
Authors: V Kafetzopou, N Kokras, I Sotiropoul, JF Oliveira, H Leite-Alme, A Vasalou, VM Sardinha, Z Papadopoul, OF Almeida, K Antoniou, N Sousa, C Dalla
Mol. Psychiatry, 2017-04-11;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC