Human/Mouse/Rat CCR5 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
Product Datasheets
Citations for Human/Mouse/Rat CCR5 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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A method for conducting suppression assays using small numbers of tissue-isolated regulatory T cells
Authors: Stephen T. Ward, Ka-Kit Li, Stuart M. Curbishley
MethodsX
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CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
Blood, 2015-02-03;125(13):2087-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
Proc Natl Acad Sci U S A, 2015-01-23;112(6):E556-65.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010-02-17;184(6):2886-98.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
Authors: Carbone T, Nasorri F, Pennino D
J. Immunol., 2009-12-11;184(2):1102-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
FASEB J., 2008-03-25;22(8):2747-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
J. Immunol., 2006-11-01;177(9):5811-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
J. Immunol., 2002-10-15;169(8):4172-82.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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Human CCR5-transfected MOLT4 cells were stained with either a PE-conjugated isotype control or the anti-CCR5 antibody. Staining was performed at room temperature for 10 minutes using the dilution and buffers stated above.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS with 2.5% Calf Serum
Dilution: 1/10
Normal rat spenocytes were stained for 10 min in room temperatures followed by 3 washes.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10
