Human/Mouse/Rat CLEC-2/CLEC1B Antibody Summary
Gln58-Pro229
Accession # AAF36777
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat CLEC-2/CLEC1B by Western Blot. Western blot shows lysates of mouse kidney tissue, rat kidney tissue, and mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CLEC-2/CLEC1B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human CLEC-2/CLEC1B by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for CLEC-2/CLEC1B at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC-2/CLEC1B
C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).
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Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
- Christou, C.M. et al. (2008) Biochem. J. 411:133.
- Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
- Suzuki-Inoue, K. et al. (2006) Blood 107:542.
- Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
- Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
- Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
- Chaipan, C. et al. (2006) J. Virol. 80:8951.
Product Datasheets
Citations for Human/Mouse/Rat CLEC-2/CLEC1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice
Authors: A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel
J Am Heart Assoc, 2017-09-22;6(9):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides
Authors: C Delierneux, N Donis, L Servais, O Wéra, C Lecut, M Vandereyke, L Musumeci, S Rahmouni, J Schneider, JA Eble, P Lancellott, C Oury
J. Thromb. Haemost, 2017-04-13;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Protein, Whole Cells
Applications: Functional Assay, Immunoprecipitation, Western Blot -
Clinicopathological correlations of podoplanin (gp38) expression in rheumatoid synovium and its potential contribution to fibroblast platelet crosstalk.
Authors: Del Rey M, Fare R, Izquierdo E, Usategui A, Rodriguez-Fernandez J, Suarez-Fueyo A, Canete J, Pablos J
PLoS ONE, 2014-06-16;9(6):e99607.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade.
Authors: Fuller GL, Williams JA, Tomlinson MG, Eble JA, Hanna SL, Pohlmann S, Suzuki-Inoue K, Ozaki Y, Watson SP, Pearce AC
J. Biol. Chem., 2007-03-05;282(17):12397-409.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
Authors: Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S
J. Virol., 2006-09-01;80(18):8951-60.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P, Neutralization
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