Human/Mouse/Rat CUGBP1/CELF1 Antibody

Catalog #: MAB9388 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9388-100
MAB9388-SP

Detection of Human, Mouse, and Rat CUGBP1/CELF1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CUGBP1/CELF1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human CUGBP1/CELF1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 850717

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CUGBP1/CELF1
Met1-Gly60
Accession # Q92879

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat CUGBP1/CELF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CUGBP1/ CELF1 Monoclonal Antibody (Catalog # MAB9388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CUGBP1/CELF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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CUGBP1/CELF1 in Human Skeletal Muscle. CUGBP1/CELF1 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat CUGBP1/CELF1 Monoclonal Antibody (Catalog # MAB9388) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CUGBP1/CELF1

CELF-1 is a highly conserved, ubiquitous protein which binds pre-mRNA in the nucleus to mediate alternative splicing to produce alternative transcripts in tissue-specific and developmentally regulated events. In the cytoplasm, CELF-1 binds to GRE-containing transcripts which are rapidly degraded. Additionally, cytoplasmic CELF-1 binds to untranslated regions of transcripts for stability and translation efficiency. Many transcripts targeted by CELF-1 show rapid up-regulation leading to cellular activation or proliferation, while others show down-regulation. CELF-1 has also been shown to localize to cytoplasmic stress granules when cells are stressed. This gene is associated with myotonic dystrophy type 1 (DM1) disease though interactions with the dystrophia myotnica-protein kinase (DMPK) gene.

Long Name

CUGBP, Elav-Like Family Member 1

Entrez Gene IDs

10658 (Human); 13046 (Mouse); 362160 (Rat)

Alternate Names

BRUNOL2; BRUNOL250 kDa nuclear polyadenylated RNA-binding protein; bruno-like 2; Bruno-like protein 2; CELF1; CUG RNA-binding protein; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; CUG-BP- and ETR-3-like factor 1; CUGBP Elav-like family member 1; CUG-BP; CUGBP, Elav-like family member 1; CUGBP1; CUG-BP1; CUGBP1CUG triplet repeat RNA-binding protein 1; CUGBPCELF-1; D2Wsu101e; Deadenylation factor CUG-BP; EDEN-BP homolog; EDEN-BP; embryo deadenylation element binding protein; Embryo deadenylation element-binding protein homolog; HNAB50; NAB50; NAB50CUG-BP1; NAPOR; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2