Human/Mouse/Rat Cyclophilin B Antibody

Catalog #: AF5410 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5410
AF5410-SP

Detection of Human/Mouse/Rat Cyclophilin B by Western Blot.

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Human/Mouse/Rat Cyclophilin B Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots and direct ELISAs, less than 1% cross-reactivity with recombinant human Cyclophilin A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cyclophilin B
Asp34-Glu216
Accession # P23284

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Knockout Validated

Cyclophilin B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cyclophilin B knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Cyclophilin B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Cyclophilin B in HeLa Human Cell Line. Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated

Cyclophilin B antibody in HeLa Human Cell Line.

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Cyclophilin B in HeLa Human Cell Line. Cyclophilin B was detected in immersion fixed wildtype (left panel) but is not detected in Cyclophilin B knockout (right panel) HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.

Entrez Gene IDs

5479 (Human); 19035 (Mouse); 64367 (Rat)

Alternate Names

Cyclophilin B; cyclophilin-like protein; CYPB; CYPBEC 5.2.1.8; CYP-S1; MGC2224; OI9MGC14109; peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase B; PPIB; Rotamase B; S-cyclophilin; SCYLP