Human/Mouse/Rat Cytochrome c Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Cytochrome c by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytochrome c at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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Detection of Human Cytochrome c by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytochrome c
Cytochrome c is a mitochondrial electron transport protein that functions in oxidative phosphorylation. Release of Cytochrome c into the cytosol is an early step in apoptotic cell death.
Product Datasheets
Citations for Human/Mouse/Rat Cytochrome c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
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Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
Mol Med Rep, 2019-06-06;20(2):879-886.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Silencing of heat shock protein 27 increases the radiosensitivity of non?small cell lung carcinoma cells
Authors: L Xu, X Lin, Y Zheng, H Zhou
Mol Med Rep, 2019-05-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming
Authors: S Sun, J Liu, M Zhao, Y Han, P Chen, Q Mo, B Wang, G Chen, Y Fang, Y Tian, J Zhou, D Ma, Q Gao, P Wu
Cell Death Dis, 2017-06-08;8(6):e2870.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
PLoS ONE, 2013-11-22;8(11):e80971.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HA14-1, a small molecule inhibitor of Bcl-2, bypasses chemoresistance in leukaemia cells.
Authors: Oliver L, Mahe B, Gréé R, Vallette FM, Juin P
Leuk. Res., 2007-01-16;31(6):859-63.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
Exp. Cell Res., 2004-08-15;298(2):560-73.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Antibody Dilution 1:100. We used this on mouse meniscus lysates. Antibody was incubated overnight and developed with ECL kit. It worked very well and we would use it again.
