Human/Mouse/Rat DEP-1/CD148 Antibody

Catalog #: AF1934
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1934
AF1934-SP

Detection of Human DEP‑1/CD148 by Western Blot.

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All DEP-1/CD148 Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat DEP-1/CD148 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DEP-1/CD148 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DEP‑1/CD148
Arg997-Ala1337
Accession # Q12913

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

2.5 µg/500 µg cell lysate

HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DEP-1/CD148 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DEP-1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta ‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain. Over aa 997-337, human Dep-1 shares 95% aa sequence identity with mouse and rat Dep-1.

References

Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
Sorby, M. et al. (2001) Oncogene 20:5219.
Jandt, E. et al. (2003) Oncogene 22:4175.
Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
Borges, L.G. et al. (1996) Circ. Res. 79:570.
de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Alternate Names

CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of

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Citations for Human/Mouse/Rat DEP-1/CD148 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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PTPRJ Inhibits Leptin Signaling, and Induction of PTPRJ in the Hypothalamus Is a Cause of the Development of Leptin Resistance
Authors: T Shintani, S Higashi, R Suzuki, Y Takeuchi, R Ikaga, T Yamazaki, K Kobayashi, M Noda
Sci Rep, 2017-09-14;7(1):11627.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor.
Authors: Spring K, Lapointe L, Caron C, Langlois S, Royal I
Cell Signal, 2014-02-28;26(6):1283-93.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
J Immunol, 2013-12-13;192(2):782-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
J. Biol. Chem., 2011-01-24;286(13):10918-29.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry