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Human/Mouse/Rat FABP5/E-FABP Antibody

Catalog #: AF1476
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1476
AF1476-SP
Detection of Human, Mouse, and Rat FABP5/E‑FABP by Western Blot.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FABP5/E-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse FABP5/E‑FABP in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FABP5/E‑FABP
Met1-Gln135
Accession # Q05816
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
Human placenta and mouse liver
Immunohistochemistry
3-15 µg/mL
See below
Knockout Validated
FABP5/E‑FABP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FABP5/E‑FABP knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat FABP5/E-FABP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP5/E‑FABP by Western Blot. Western blot shows lysates of human placenta tissue, mouse liver tissue, rat brain tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FABP5/E-FABP antibody in Mouse Skin Tissue by Immunohistochemistry (IHC-P). View Larger

FABP5/E‑FABP in Mouse Skin Tissue. FABP5/E-FABP was detected in immersion fixed paraffin-embedded sections of mouse skin (ear) tissue using Goat Anti-Human/Mouse/Rat FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Human and Mouse FABP5/E‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human placenta and mouse liver, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 20 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP5/E‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Knockout Validated Western Blot Shows Human FABP5/E-FABP Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FABP5/E‑FABP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1476) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP5/E-FABP

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Long Name
Fatty Acid-Binding Protein 5
Entrez Gene IDs
2171 (Human); 16592 (Mouse); 140868 (Rat)
Alternate Names
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog

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Citations for Human/Mouse/Rat FABP5/E-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erb?/FABP5 signaling
    Authors: Zhong, D;Chen, J;Qiao, R;Song, C;Hao, C;Zou, Y;Bai, M;Su, W;Yang, B;Sun, D;Jia, Z;Sun, Y;
    Cell reports
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Fatty Acid-Binding Protein 5 Modulates Brain Endocannabinoid Tone and Retrograde Signaling in the Striatum
    Authors: M Fauzan, S Oubraim, M Yu, ST Glaser, M Kaczocha, S Haj-Dahman
    Frontiers in Cellular Neuroscience, 2022-07-07;16(0):936939.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain
    Authors: DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha
    Oncogene, 2022-06-02;12(1):9241.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Stroke induces disease-specific myeloid cells in the brain parenchyma and pia
    Authors: C Beuker, D Schafflick, JK Strecker, M Heming, X Li, J Wolbert, A Schmidt-Po, C Thomas, T Kuhlmann, I Aranda-Par, N A-Gonzalez, PA Kumar, Y Werner, E Kilic, DM Hermann, H Wiendl, R Stumm, GMZ Hörste, J Minnerup
    Nature Communications, 2022-02-17;13(1):945.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Destructing biofilms by cationic dextran through phase transition
    Authors: Y Li, S Wang, Z Xing, Y Niu, Z Liao, Y Lu, J Qiu, J Zhang, C Wang, L Dong
    Carbohydrate polymers, 2021-10-23;279(0):118778.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin
    Authors: K Singh, E Camera, L Krug, A Basu, RK Pandey, S Munir, M Wlaschek, S Kochanek, M Schorpp-Ki, M Picardo, P Angel, C Niemann, P Maity, K Scharffett
    Nat Commun, 2018-08-24;9(1):3425.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide Production
    Authors: Yuwen Zhang
    J. Immunol, 2016-12-05;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.
    Authors: Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, Natsuga K, Lin K, Watt F
    Stem Cell Reports, 2014-09-18;3(4):620-33.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
    Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
    Cancer Res, 2014-04-08;74(11):2986-98.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: IHC, Western Blot
  10. Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain.
    Authors: Yu S, Levi L, Casadesus G, Kunos G, Noy N
    J Biol Chem, 2014-03-18;289(18):12748-58.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta).
    Authors: Yu S, Levi L, Siegel R, Noy N
    J Biol Chem, 2012-10-26;287(50):42195-205.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Western Blot
  12. Proteomic analysis of proteins differentially expressed in uterine lymphocytes obtained from wild-type and NOD mice.
    Authors: Li C, Wang W, Wang H, Zhong Y, Di J, Lin Y
    J. Cell. Biochem., 2009-10-01;108(2):447-57.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. All-trans-retinoic acid represses obesity and insulin resistance by activating both peroxisome proliferation-activated receptor beta/delta and retinoic acid receptor.
    Authors: Berry DC, Noy N
    Mol. Cell. Biol., 2009-04-13;29(12):3286-96.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Helper-dependent adenovirus-mediated short hairpin RNA expression in the liver activates the interferon response.
    Authors: Witting SR, Brown M, Saxena R, Nabinger S, Morral N
    J. Biol. Chem., 2007-11-19;283(4):2120-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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