Human/Mouse/Rat GAD1/GAD67 Antibody

Catalog #: AF2086
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2086
AF2086-SP

Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot.

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All GAD1/GAD67 Antibodies

Product Details

Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GAD1/GAD67 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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GAD1/GAD67 in Human Spinal Cord. GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Mouse GAD1/GAD67 by Simple Western^TM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD1/GAD67

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Entrez Gene IDs

2571 (Human)

Alternate Names

67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat GAD1/GAD67 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies
Authors: Song, Y;Seward, CH;Chen, CY;LeBlanc, A;Leddy, AM;Stubbs, L;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease
Authors: S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu
Acta neuropathologica communications, 2022-12-21;10(1):188.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
The beta2V287L nicotinic subunit linked to sleep-related epilepsy differently affects fast-spiking and regular spiking somatostatin-expressing neurons in murine prefrontal cortex
Authors: S Meneghini, D Modena, G Colombo, A Coatti, N Milani, L Madaschi, A Amadeo, A Becchetti
Progress in neurobiology, 2022-05-02;0(0):102279.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Regulation of prefrontal patterning and connectivity by retinoic acid
Authors: M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan
Nature, 2021-10-01;0(0):.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC
Alterations in a Unique Class of Cortical Chandelier Cell Axon Cartridges in Schizophrenia
Authors: Kenneth N Fish
Biol. Psychiatry, 2016-09-29;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis.
Authors: Mayerl S, Muller J, Bauer R, Richert S, Kassmann C, Darras V, Buder K, Boelen A, Visser T, Heuer H
J Clin Invest, 2014-04-01;124(5):1987-99.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Locomotor deficiencies and aberrant development of subtype-specific GABAergic interneurons caused by an unliganded thyroid hormone receptor alpha1.
Authors: Wallis K, Sjogren M, van Hogerlinden M, Silberberg G, Fisahn A, Nordstrom K, Larsson L, Westerblad H, Morreale de Escobar G, Shupliakov O, Vennstrom B
J. Neurosci., 2008-02-20;28(8):1904-15.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr