Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody

Catalog #: MAB5470
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5470
MAB5470-SP

Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot.

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All Glutathione Peroxidase 2/GPX2 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat GPX2 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 496010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Glutathione Peroxidase 2
Gly41-Ile190
Accession # P18283

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot

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Detection of Rat Glutathione Peroxidase 2/GPX2 by Western Blot Expression of various ROS-related proteins, pro-inflammatory factors, and an osteoclast differentiation factor RANKL in tissue samples of all treated groups at 24 weeks after surgery.(A) Representative Western blot analysis of transferrin, catalase, SOD1, SOD2, SOD3, GPx1, GPx2, TTR, NF-kappa B, IL-1 beta and RANKL in different groups. GAPDH was used as internal control. (B) Densitometric quantification of Western blot analysis for all tested proteins normalized to GAPDH (n = 3 for each group). *, P < 0.05, for results compared to those of the sham group; #, P < 0.05, for results compared to those of the group receiving an artificial knee joint only. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30125327), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 2/GPX2

Glutathione Peroxidase 2 (GPX2), also known as Gastrointestinal Glutathione Peroxidase, is a member of the glutathione peroxidase antioxidant enzyme family. These proteins protect cell surfaces, extracellular fluid components, and enzymes from oxidative stress by reducing hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX2 is a cytoplasmic homotetramer of 22 kDa subunits, each containing a selenocysteine residue at the active site. GPX2 is primarily expressed in the liver and intestinal epithelial cells where it participates in the detoxification of dietary organic hydroperoxides. It is upregulated in a variety of carcinomas and functions as an anti-inflammatory and anti-apoptotic molecule. Over amino acids 41‑190, human GPX2 shares 94% aa sequence identity with mouse and rat GPX2.

Entrez Gene IDs

2877 (Human); 14776 (Mouse); 29326 (Rat)

Alternate Names

EC 1.11.1; EC 1.11.1.9; gastrointestinal glutathione peroxidase 2; Gastrointestinal glutathione peroxidase; GIGPX; GI-GPx; glutathione peroxidase 2 (gastrointestinal); Glutathione Peroxidase 2; Glutathione peroxidase-gastrointestinal; Glutathione peroxidase-related protein 2; GPRP; GPRP-2; GPX2; GPx-2; GPx-GI; GSHPx-2; GSHPX-GI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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IRON-MEDIATED EPIGENETIC ACTIVATION OF NRF2 TARGETS
Authors: RD Horniblow, P Pathak, DL Balacco, A Acharjee, E Lles, G Gkoutos, AD Beggs, C Tselepis
The Journal of nutritional biochemistry, 2021-12-23;0(0):108929.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis
Authors: KT Peng, MH Tsai, CW Lee, YC Chiang, PC Chen, CC Chen, CH Chang, HN Shih, PJ Chang
PLoS ONE, 2018-08-20;13(8):e0202501.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Sigma 1 receptor regulates the oxidative stress response in primary retinal Müller glial cells via NRF2 signaling and system xc(-), the Na(+)-independent glutamate-cystine exchanger
Authors: Jing Wang, Arul Shanmugam, Shanu Markand, Eric Zorrilla, Vadivel Ganapathy, Sylvia B. Smith
Free Radical Biology and Medicine