Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Antibody
Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Antibody Summary
Gly74-Lys226
Accession # P46412
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Glutathione Peroxidase 3 by Western Blot. Western blot shows lysates of human plasma, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3 at approximately 24kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Glutathione Peroxidase 3/GPX3 in Human Kidney. Glutathione Peroxidase 3/GPX3 was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Glutathione Peroxidase 3/GPX3 by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 29 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4199) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione Peroxidase 3/GPX3
Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.
Product Datasheets
Citations for Human/Mouse/Rat
Glutathione Peroxidase 3/GPX3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Analysis of Bioavailability and Induction of Glutathione Peroxidase by Dietary Nanoelemental, Organic and Inorganic Selenium
Authors: MT Ringuet, B Hunne, M Lenz, DM Bravo, JB Furness
Nutrients, 2021-03-25;13(4):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress.
Authors: Van Brussel, Ilse, Schrijvers, Dorien M, Martinet, Wim, Pintelon, Isabel, Deschacht, Maartje, Schnorbusch, Kathy, Maes, Louis, Bosmans, Johan M, Vrints, Christia, Adriaensen, Dirk, Cos, Paul, Bult, Hidde
PLoS ONE, 2012-08-15;7(8):e43357.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice.
Authors: Seale L, Hashimoto A, Kurokawa S, Gilman C, Seyedali A, Bellinger F, Raman A, Berry M
Mol Cell Biol, 2012-08-13;32(20):4141-54.
Species: Mouse
Sample Types: Serum
Applications: Western Blot -
A role for GPx3 in activity of normal and leukemia stem cells.
Authors: Herault O, Hope KJ, Deneault E, Mayotte N, Chagraoui J, Wilhelm BT, Cellot S, Sauvageau M, Andrade-Navarro MA, Hebert J, Sauvageau G
J. Exp. Med., 2012-04-16;209(5):895-901.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Glutathione Peroxidase 3/GPX3 Antibody
Heat-induced antigen retrieval with citrate buffer
antibody concentration 1:400
