Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody

Catalog #: BAF4199 Datasheet / COA / SDS

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BAF4199

Detection of Human Glutathione Peroxidase 3/GPX3 by Western Blot.

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Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Glutathione Peroxidase 3/GPX3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Glutathione Peroxidase 3/GPX3
Gly74-Lys226
Accession # P46412

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Glutathione Peroxidase 3/GPX3 by Western Blot. Western blot shows human serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 3/GPX3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 3/GPX3 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 3/GPX3

Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively.

Entrez Gene IDs

2878 (Human); 14778 (Mouse); 64317 (Rat)

Alternate Names

EC 1.11.1; EC 1.11.1.9; Extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Glutathione Peroxidase 3; GPX3; GPx-3; GPXP; GPX-P; GSHPx-3; GSHPx-P; Plasma glutathione peroxidase