Human/Mouse/Rat Glyoxalase I Antibody

Catalog #: AF4959 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4959
AF4959-SP

Detection of Human/Mouse/Rat Glyoxalase I by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Glyoxalase I Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Glyoxalase I in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Glyoxalase I
Ala2-Met184
Accession # Q04760

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Glyoxalase I by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glyoxalase I at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human Glyoxalase I by Simple Western^TM Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glyoxalase I at approximately 33 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4959) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glyoxalase I

Glyoxalase I (also lactoylglutathione lyase, methylglyoxalase, and glx I) is a 21 kDa member of the Glyoxalase I family. The enzyme is an isomerase that catalyzes the formation of S-D-lactoylglutathione from the hemimercaptal adduct that forms spontaneously between methylglyoxal and reduced GSH (1‑4). The monomeric subunit for human Glyoxalase I is 184 amino acids (aa) in length. In the mature protein, the methionine at the N-terminus is removed. Human Glyoxalase I exists in three separable isoforms as homo-and hetero-dimers of two allelic subunit variants, which differ in charge (1). The isoforms are formed when residue 19 is changed from cysteine to tyrosine and residue 111 is changed from glutamine to alanine. Each subunit binds one Zn²⁺ atom (1, 3‑4). The protein is made up of multiple beta strands and alpha helical regions. Human Glyoxalase I shares 91% and 90% aa sequence identity with rat and mouse Glyoxalase I, respectively. The enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated (1). The biological role of the enzyme remains unclear, but the glyoxalase system detoxifies the precursors of advanced glycation end products, which take part in the pathogenesis of vascular, diabetic, and uremic complications (5).

References

Ridderstrom, M. & B. Mannervik (1996) Biochem. J. 314:463.
Marmstal, E. & B. Mannervik (1981) FEBS Lett. 131:301.
Kim, N-S. et al. (1993) J. Biol. Chem. 268:11217.
Ranganathan, S. et al. (1993) J. Biol. Chem. 268:5661.
Kalousova, M. et al. (2007) Ann. N. Y. Acad. Sci. 1126:268.

Entrez Gene IDs

2739 (Human); 109801 (Mouse); 294320 (Rat)

Alternate Names

Aldoketomutase; EC 4.4.1.5; GLO1; GLOD1; Glx I; GLYI; glyoxalase domain containing 1; Glyoxalase I; glyoxalase Ialdoketomutase; Ketone-aldehyde mutase; lactoyl glutathione lyase; lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase