Human/Mouse/Rat Histamine H3 R Antibody

Catalog #: MAB102001 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB102001-100
MAB102001-SP

Detection of HRH3 in HEK293 Human Cell Line Transfected with Human HRH3 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Histamine H3 R Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Histamine H3 R in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2420B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing Human/Mouse/Rat Histamine H3 R
Accession # Q9Y5N1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of HRH3 in HEK293 Human Cell Line Transfected with Human HRH3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human HRH3 or (B) irrelevant protein, and EGFP, was stained with Rabbit Anti-Human HRH3 Monoclonal Antibody (Catalog # MAB102001) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine H3 R

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors Histamine H1 R, Histamine H2 R, Histamine H3 R and Histamine H4 R. HRH3 encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. Histamine H3 R is an integral membrane protein and can regulate neurotransmitter release. It can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.

Long Name

Histamine H3 Receptor

Entrez Gene IDs

11255 (Human); 99296 (Mouse)

Alternate Names

GPCR97; GPCR97G protein-coupled receptor 97; G-protein coupled receptor 97; H3R; HH3R; Histamine H3 R; histamine H3 receptor; Histamine H3R; histamine receptor H3; HRH3