Human/Mouse/Rat HSPB8 Antibody Summary
Ala2-Thr196
Accession # Q9UJY1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human, Mouse, and Rat HSPB8 by Western Blot. Western blot shows lysates of human, mouse, and rat heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPB8 Monoclonal Antibody (Catalog # MAB4987) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPB8 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSPB8
Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22‑25 kDa member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha -crystalline domain (aa 93‑170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A.
Product Datasheets
Citations for Human/Mouse/Rat HSPB8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy
Authors: R Cristofani, M Piccolella, M Montagnani, B Tedesco, A Poletti, RM Moretti
Cell Death & Disease, 2022-11-18;13(11):973.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Correlation between the complex of small heat shock proteins (HSPBs) and the progression in patients with hepatocellular carcinoma
Authors: R Matsushima, H Toyoda, M Atsuyuki, Y Kaneoka, T Kumada, O Kozawa
Archives of biochemistry and biophysics, 2022-11-05;732(0):109461.
Species: Human
Sample Types: Tissue Homogenates
Applications: Co-Immunoprecipitation, Western Blot -
HSPB8 Promotes the Fusion of Autophagosome and Lysosome during Autophagy in Diabetic Neurons
Authors: XC Li, QK Hu, L Chen, SY Liu, S Su, H Tao, LN Zhang, T Sun, LJ He
Int J Med Sci, 2017-10-15;14(13):1335-1341.
Species: Human, Rat
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat HSPB8 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat HSPB8 Antibody and earn rewards!
Have you used Human/Mouse/Rat HSPB8 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
