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Human/Mouse/Rat HTRA2/Omi Antibody

Catalog #: AF1458
23 Citations Datasheet / COA / SDS
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AF1458
AF1458-SP
Detection of Human/Mouse/Rat HTRA2/Omi by Western Blot.
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Citations (23)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HTRA2/Omi Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat full length and mitochondria-processed HTRA2/Omi.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HTRA2/Omi
Ala134-Glu458
Accession # O43464
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat HTRA2/Omi antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat HTRA2/Omi by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HTRA2/Omi at approximately 36 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry HTRA2/Omi antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

HTRA2/Omi in Jurkat Human Cell Line. HTRA2/Omi was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human and Mouse HTRA2/Omi antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse HTRA2/Omi by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for HTRA2/Omi at approximately 51 kDa (precursor) and 41 kDa (processed) (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HTRA2/Omi

HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.

References
  1. Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
  2. van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
  3. Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
  4. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
  5. Martins, L. et al. (2002) J. Biol. Chem. 277:439.
  6. Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
  7. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.
Long Name
High Temperature Requirement Protein-2
Entrez Gene IDs
27429 (Human); 64704 (Mouse)
Alternate Names
High temperature requirement protein A2; HtrA serine peptidase 2; HTRA2; HtrA-like serine protease; Omi; OMIOmi stress-regulated endoprotease; PARK13EC 3.4.21.108; PRSS25; PRSS25serine, 25; Serine protease 25; serine protease HTRA2, mitochondrial; Serine proteinase OMI

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Citations for Human/Mouse/Rat HTRA2/Omi Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytes
    Authors: R Aishwarya, S Alam, CS Abdullah, M Morshed, SS Nitu, M Panchatcha, S Miriyala, CG Kevil, MS Bhuiyan
    Redox Biol, 2020-07-26;36(0):101660.
    Species: Rat
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  2. Cytosolic Trapping of a Mitochondrial Heat Shock Protein Is an Early Pathological Event in Synucleinopathies
    Authors: ÉM Szeg?, A Dominguez-, E Gerhardt, A König, DJ Koss, W Li, R Pinho, C Fahlbusch, M Johnson, P Santos, A Villar-Piq, T Thom, S Rizzoli, M Schmitz, J Li, I Zerr, J Attems, O Jahn, TF Outeiro
    Cell Rep, 2019-07-02;28(1):65-77.e6.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis
    Authors: S Saita, H Nolte, KU Fiedler, H Kashkar, AS Venne, RP Zahedi, M Krüger, T Langer
    Nat. Cell Biol, 2017-03-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Protease Omi cleaving Hax-1 protein contributes to OGD/R-induced mitochondrial damage in neuroblastoma N2a cells and cerebral injury in MCAO mice.
    Authors: Wu J, Li M, Cao L, Sun M, Chen D, Ren H, Xia Q, Tao Z, Qin Z, Hu Q, Wang G
    Acta Pharmacol Sin, 2015-08-24;36(9):1043-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Protease Omi facilitates neurite outgrowth in mouse neuroblastoma N2a cells by cleaving transcription factor E2F1.
    Authors: Ma Q, Hu Q, Xu R, Zhen X, Wang G
    Acta Pharmacol Sin, 2015-08-01;36(8):966-75.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  6. Heat shock inhibition of CDK5 increases NOXA levels through miR-23a repression.
    Authors: Morey T, Roufayel R, Johnston D, Fletcher A, Mosser D
    J Biol Chem, 2015-03-31;290(18):11443-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Neural-specific deletion of Htra2 causes cerebellar neurodegeneration and defective processing of mitochondrial OPA1.
    Authors: Patterson, Victoria, Zullo, Alfred J, Koenig, Claire, Stoessel, Sean, Jo, Hakryul, Liu, Xinran, Han, Jinah, Choi, Murim, DeWan, Andrew T, Thomas, Jean-Leo, Kuan, Chia-Yi, Hoh, Josephin
    PLoS ONE, 2014-12-22;9(12):e115789.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. PINK1 kinase catalytic activity is regulated by phosphorylation on serines 228 and 402.
    Authors: Aerts L, Craessaerts K, de Strooper B, Morais V
    J Biol Chem, 2014-12-19;290(5):2798-811.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Influenza A virus protein PB1-F2 translocates into mitochondria via Tom40 channels and impairs innate immunity.
    Authors: Yoshizumi T, Ichinohe T, Sasaki O, Otera H, Kawabata S, Mihara K, Koshiba T
    Nat Commun, 2014-08-20;5(0):4713.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The ubiquitin-conjugating enzymes UBE2N, UBE2L3 and UBE2D2/3 are essential for Parkin-dependent mitophagy.
    Authors: Geisler S, Vollmer S, Golombek S, Kahle P
    J Cell Sci, 2014-06-06;127(0):3280-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion.
    Authors: Yamauchi S, Hou Y, Guo A, Hirata H, Nakajima W, Yip A, Yu C, Harada I, Chiam K, Sawada Y, Tanaka N, Kawauchi K
    J Cell Biol, 2014-03-24;204(7):1191-207.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  12. The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria.
    Authors: Jin, Seok Min, Youle, Richard
    Autophagy, 2013-09-05;9(11):1750-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. PINK1 is degraded through the N-end rule pathway.
    Authors: Yamano, Koji, Youle, Richard
    Autophagy, 2013-04-17;9(11):1758-69.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.
    Authors: Geisler S, Holmstrom KM, Skujat D, Fiesel FC, Rothfuss OC, Kahle PJ, Springer W
    Nat. Cell Biol., 2010-01-24;12(2):119-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders.
    Authors: Johnson F, Kaplitt MG
    PLoS ONE, 2009-09-18;4(9):e7100.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration.
    Authors: Ding X, Patel M, Shen D, Herzlich AA, Cao X, Villasmil R, Klupsch K, Tuo J, Downward J, Chan CC
    Invest. Ophthalmol. Vis. Sci., 2009-05-14;50(10):4957-66.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  17. Identification of a novel protein MICS1 that is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c.
    Authors: Oka T, Sayano T, Tamai S, Yokota S, Kato H, Fujii G, Mihara K
    Mol. Biol. Cell, 2008-04-16;19(6):2597-608.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation.
    Authors: Huttunen HJ, Guenette SY, Peach C, Greco C, Xia W, Kim DY, Barren C, Tanzi RE, Kovacs DM
    J. Biol. Chem., 2007-08-06;282(38):28285-95.
    Species: Hamster
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  19. Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
    Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
    Apoptosis, 2006-11-01;11(11):1959-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  20. Induction of BIM(EL) following growth factor withdrawal is a key event in caspase-dependent apoptosis of 661W photoreceptor cells.
    Authors: Gomez-Vicente V, Doonan F, Donovan M, Cotter TG
    Eur. J. Neurosci., 2006-08-01;24(4):981-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
    Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
    Mol. Endocrinol., 2006-05-18;20(10):2469-82.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Roscovitine-induced up-regulation of p53AIP1 protein precedes the onset of apoptosis in human MCF-7 breast cancer cells.
    Authors: Wesierska-Gadek J, Gueorguieva M, Horky M
    Mol. Cancer Ther., 2005-01-01;4(1):113-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Motoneuron resistance to apoptotic cell death in vivo correlates with the ratio between X-linked inhibitor of apoptosis proteins (XIAPs) and its inhibitor, XIAP-associated factor 1.
    Authors: Perrelet D, Perrin FE, Liston P, Korneluk RG, MacKenzie A, Ferrer-Alcon M, Kato AC
    J. Neurosci., 2004-04-14;24(15):3777-85.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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