Human/Mouse/Rat iNOS Antibody Summary
Pro781-His798
Accession # P35228
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human iNOS by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
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Detection of Mouse and Rat iNOS by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Mouse iNOS by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: iNOS
Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.
Product Datasheets
Citations for Human/Mouse/Rat iNOS Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Synthesis of Carvone Derivatives and In Silico and In Vitro Screening of Anti-Inflammatory Activity in Murine Macrophages
Authors: G Moço, C Sousa, A Capitão, SS MacKinnon, AJ Leitão, AF Mendes
International Journal of Molecular Sciences, 2023-01-23;24(3):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells
Authors: B Saravi, Z Li, V Basoli, S Grad, S Häckel, CE Albers, M Alini, H Schmal, P Obid, G Lang
Cells, 2022-10-28;11(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis
Authors: JY Hong, H Kim, J Lee, WJ Jeon, YJ Lee, IH Ha
Oxidative Medicine and Cellular Longevity, 2022-09-14;2022(0):3472443.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Insulin-like growth factor 2 promotes osteoclastogenesis increasing inflammatory cytokine levels under hypoxia
Authors: T Kondo, H Aoki, Y Otsuka, Y Kawaguchi, Y Waguri-Nag, M Aoyama
Journal of pharmacological sciences, 2022-04-16;149(3):93-99.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury
Authors: YR Hong, EH Lee, KS Park, M Han, KT Kim, J Park
Scientific Reports, 2022-03-07;12(1):3636.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
Authors: F Yu, W Tian, J Dong
International immunopharmacology, 2022-01-16;104(0):108514.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gongjin-Dan Enhances Neurite Outgrowth of Cortical Neuron by Ameliorating H2O2-Induced Oxidative Damage via Sirtuin1 Signaling Pathway
Authors: H Kim, W Jeon, J Hong, J Lee, C Yeo, Y Lee, S Baek, I Ha
Nutrients, 2021-11-27;13(12):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Senolytic Therapy for Cerebral Ischemia-Reperfusion Injury
Authors: S Lim, TJ Kim, YJ Kim, C Kim, SB Ko, BS Kim
International Journal of Molecular Sciences, 2021-11-04;22(21):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice
Authors: Y Wang, H Jin, Y Wang, Y Yao, C Yang, J Meng, X Tan, Y Nie, L Xue, B Xu, H Zhao, F Wang
Theranostics, 2021-11-01;11(20):10074-10090.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP?1 cells via the Rb/E2F1 pathway in cervical carcinoma
Authors: L Liu, W Shi, X Xiao, X Wu, H Hu, S Yuan, K Liu, Z Liu
Oncology reports, 2021-09-28;46(5):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Migration and phenotype switching of macrophages at early-phase of bone-formation by secretomes from bone marrow derived mesenchymal stem cells using rat calvaria bone defect model
Authors: W Katagiri, R Takeuchi, N Saito, D Suda, T Kobayashi
Journal of dental sciences, 2021-08-31;17(1):421-429.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Neurotherapeutic Potential of Cervus elaphus Sibericus on Axon Regeneration and Growth Cone Reformation after H2O2-Induced Injury in Rat Primary Cortical Neurons
Authors: JY Hong, J Lee, H Kim, WJ Jeon, C Yeo, BR Choi, JE Yoon, JY Shin, JY Kim, IH Ha
Biology, 2021-08-26;10(9):.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface
Authors: A Chu, SY Kok, J Tsui, MC Lin, B Aguirre, M Wadehra
Journal of reproductive immunology, 2021-03-09;145(0):103309.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis
Authors: C Vaamonde-G, N Flórez-Fer, MD Torres, MJ Lamas-Vázq, FJ Blanco, H Domínguez, R Meijide-Fa
Carbohydrate polymers, 2021-01-26;258(0):117692.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-inflammatory potential of Portuguese thermal waters
Authors: A Silva, AS Oliveira, CV Vaz, S Correia, R Ferreira, L Breitenfel, J Martinez-d, R Palmeira-d, CMF Pereira, A Palmeira-d, MT Cruz
Scientific Reports, 2020-12-18;10(1):22313.
Species: Mouse
Sample Types: Cell Lysates
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Cancer-Derived Transforming Growth Factor-beta Modulates Tumor-Associated Macrophages in Ampullary Cancer
Authors: LC Cheng, YJ Chao, CY Wang, NN Phan, YL Chen, TW Wang, HP Hsu, YJ Lin, YS Shan, MD Lai
Onco Targets Ther, 2020-08-03;13(0):7503-7516.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Standardised comparison of limonene-derived monoterpenes identifies structural determinants of anti-inflammatory activity
Authors: C Sousa, AJ Leitão, BM Neves, F Judas, C Cavaleiro, AF Mendes
Sci Rep, 2020-04-29;10(1):7199.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Giardia lamblia Decreases NF-&kappaB p65RelA Protein Levels and Modulates LPS-Induced Pro-Inflammatory Response in Macrophages
Authors: CP Faria, BM Neves, Á Lourenço, MT Cruz, JD Martins, A Silva, S Pereira, MDC Sousa
Sci Rep, 2020-04-10;10(1):6234.
Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot -
Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model
Authors: WC Liu, PH Chen, LW Chen
J. Nutr. Biochem., 2020-04-01;83(0):108384.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes
Authors: MAM Simões, DCGA Pinto, BMR Neves, AMS Silva
Molecules, 2020-02-13;25(4):.
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western Blot -
The pro-inflammatory effect of NR4A3 in osteoarthritis
Authors: C Ma, L Wu, L Song, Y He, S Adel Abdo, S Yan, K Sheng, H Wu, J Ran, L Wu
J. Cell. Mol. Med., 2019-11-07;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery
Authors: W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu
J. Exp. Clin. Cancer Res., 2019-02-08;38(1):62.
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western Blot -
Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns
Authors: S Altan, Z O?urtan
Burns, 2016-10-27;43(1):232-244.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction.
Authors: Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M
PLoS ONE, 2015-04-15;10(4):e0122458.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.
Authors: Xia N, Pautz A, Wollscheid U, Reifenberg G, Forstermann U, Li H
Molecules, 2014-03-24;19(3):3654-68.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy.
Authors: Dietrich S, Okun J, Schmidt K, Falk C, Wagner A, Karamustafa S, Radujkovic A, Hegenbart U, Ho A, Dreger P, Luft T
Haematologica, 2013-10-18;99(3):541-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
BMC Neurosci, 2012-05-23;13(0):50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.
Authors: Espinosa A, Oke V, Elfving A, Nyberg F, Covacu R, Wahren-Herlenius M
Exp. Cell Res., 2008-09-25;314(20):3605-13.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Authors: Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
Infect. Immun., 2006-10-02;74(12):7010-3.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production.
Authors: Yuan LJ, Ueng SW, Lin SS, Yeh WL, Yang CY, Lin PY
J. Orthop. Res., 2004-09-01;22(5):1126-34.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P
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Antibody also works very well for immunohistochemistry
Rats were perfused with 4% paraformaldehyde in PBS (pH=7.6). The brain was removed and immersed in PFA then in 30% sucrose. The brain was sliced with a freezing microtome. A mixture of 1% Triton-X and 10% donkey serum (in TRIS) was used for antigen retrieval and blocking.
Tmem119 antibody was used in 1:100 dilution and was visualized by a Cy3 conjugated anti-mouse secondary antibody (1:500, yellow). Cell bodies were stained with NeuroTrace 640/660 (1:250, red). Pictures show a part of the rat hippocampus.
The product was assumed to label activated microglial cells. It seems, it did not. However, it labelled some cerebral blood vessels, which is also meaningful, thus, it may be useful for this application.
