Human/Mouse/Rat Intelectin-1/2 Antibody

Catalog #: MAB42542 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB42542-100
MAB42542-SP

Detection of Human, Mouse, and Rat Intelectin-1/2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Intelectin-1/2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Intelectin-1 and human Intelectin-2 in direct ELISAs. Detects human, mouse, and rat Intelectin-1 and Intelectin-2 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 450515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human Intelectin-1
Thr19-Ser298
Accession # Q8WWA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

0.5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Intelectin-1/2 by Western Blot. Western blot shows lysates of recombinant human Intelectin-1 (Catalog # 9137-IN), recombinant human Intelectin-2, human small intestine tissue, mouse small intestine tissue, and rat small intestine tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin-1/2 at approximately 36-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Intelectin‑1/2 in Human Small Intestine. Intelectin-1/2 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Intelectin‑1/2 in Human Ileum Air-Liquid Interface. Intelectin-1/2 was detected in immersion fixed paraffin-embedded sections of human ileum air-liquid interface using Mouse Anti-Human/Mouse Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mucus granules in goblet cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/2

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition. The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7). It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 44-267). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.

Entrez Gene IDs

55600 (Human)

Alternate Names

Intelectin-1 / Intelectin-2; Intelectin-1/2