Human/Mouse/Rat Keap1 Antibody Summary
Ala90-Ile250
Accession # Q14145
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Keap1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
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Detection of Human Keap1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Keap1 at approximately 75 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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Western Blot Shows Human Keap1 Specificity by Using Knockout Cell Line. Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Keap1
Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.
Product Datasheets
Citations for Human/Mouse/Rat Keap1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion
Authors: H Tendilla-B, S Meneses-Pr, R Antonio Vá, M Tapia-Rodr, A Judith Váz, H Coatl-Cuay, D Martín-Her, KS MacDowell, L Garcés-Ram, JC Leza, G Flores
J. Neurosci., 2019-09-13;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Nrf2-Keap1 pathway promotes cell proliferation and diminishes ferroptosis
Authors: Z Fan, AK Wirth, D Chen, CJ Wruck, M Rauh, M Buchfelder, N Savaskan
Oncogenesis, 2017-08-14;6(8):e371.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
Authors: Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, Yehuda-Shnaidman E, Lee C, Lau J, Kurtz TW, Chan JY
J. Biol. Chem., 2010-01-20;285(12):9292-300.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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