Human/Mouse/Rat LC3B Antibody Summary
Accession # Q9GZQ8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Western Blot Show Human LC3B Specificity Using Knockout Cell Line. Western Blot Show Human LC3B Specificity Using Knockout Cell Line.](https://resources.rndsystems.com/images/datasheets/antibody/LC3B_MAB85582_Knockout_Validated_21293.jpg)
Western Blot Show Human LC3B Specificity Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of Human LC3B antibody by Western Blot. Detection of Human LC3B antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/LC3A_MAB85582_Western_Blot_21199.jpg)
Detection of Human LC3B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 Hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of Mouse and Rat LC3B antibody by Western Blot. Detection of Mouse and Rat LC3B antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/LC3B_MAB85582_Western_Blot_21292.jpg)
Detection of Mouse and Rat LC3B by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![LC3B antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). LC3B antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/LC3A_MAB85582_Immunocytochemistry__Immunofluorescence_21140.jpg)
LC3B in HeLa Human Cell Line. LC3B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Chloroquine using Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
![Detection of Mouse LC3B antibody by Simple Western<sup>TM</sup>. Detection of Mouse LC3B antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/LC3B_MAB85582_Simple_Western_21298.jpg)
Detection of Mouse LC3B by Simple WesternTM. Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LC3B
Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy.
- Shpilka, T. et al. (2011) Genome Biol. 12:226.
- He, H. et al. (2003) J. Biol. Chem. 278:29278.
- Kuznetsov, S.A. & V.I. Gelfand (1987) FEBS Let. 212:145.
- Weidberg, H. et al. (2011) Ann Rev. Biochem. 80:125.
- Weidberg, H. et al. (2010) EMBO J. 29:1792.
Product Datasheets
Citation for Human/Mouse/Rat LC3B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Targeted Lipid Nanoparticles Encapsulating Dihydroartemisinin and Chloroquine Phosphate for Suppressing the Proliferation and Liver Metastasis of Colorectal Cancer
Authors: J Peng, Q Wang, J Zhou, S Zhao, P Di, Y Chen, L Tao, Q Du, X Shen, Y Chen
Frontiers in Pharmacology, 2021-10-08;12(0):720777.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat LC3B Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat LC3B Antibody and earn rewards!
Have you used Human/Mouse/Rat LC3B Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image