Human/Mouse/Rat Lipocalin-2/NGAL Antibody Summary
Gln21-Gly198
Accession # P80188
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse and Rat Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of mouse and rat bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/ Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin-2/NGAL at approximately 34 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human Lipocalin-2/NGAL by Western Blot Enhanced expression of antimicrobial effectors by HO53 and HO56 stimulation in BCi cells. Samples were collected after 24 h (black bars) and 48 h (grey bars) post stimulation with Entinostat (10 μM), HO53 (75 μM) and HO56 (75 μM). Followed by expression analysis at mRNA and protein level by qRT-PCR and ELISA/Western blot, respectively. (a) Expression of LCN2 (lipocalin 2) at mRNA and (b) protein (lipocalin 2, NGAL) level in cell lysates. The representative Western blot is selected from one of the 3 independent experiments. GAPDH was used as a loading control. Full-length blots are presented in Supplementary Figure S10b. (c) Fold change of HBD1 (human beta -defensin 1) mRNA in comparison to control (Ctrl) and (d) secretion of hBD-1 peptide measured by ELISA in cell culture supernatants. (e) S100A8 expression was analyzed by qRT-PCR. TUBB (tubulin-beta ) was the reference gene in qRT-PCR. Each bar represents mean value of 3 independent experiments ± SEM; statistical significance was calculated in comparison to the control group using two-way ANOVA with Dunnett’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Significant changes are highlighted. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31068616), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells. Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.
Product Datasheets
Citations for Human/Mouse/Rat Lipocalin-2/NGAL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
37
Citations: Showing 1 - 10
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miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity
Authors: Liu HH, Li XQ, Liu JF et al.
Frontiers in Medicine
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Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
Authors: Kovacevic S, Ivanov M, Zivotic M Et al.
Antioxidants (Basel)
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Expression of ACE2 in the Intact and Acutely Injured Kidney
Authors: Nath K, Singh R, Grande J et al.
Kidney360
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Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma
Authors: Asimakopoulou A, Vucur M, Luedde T et al.
Cancers (Basel)
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Human conjunctiva organoids to study ocular surface homeostasis and disease
Authors: Bannier-Hélaouët, M;Korving, J;Ma, Z;Begthel, H;Giladi, A;Lamers, MM;van de Wetering, WJ;Yawata, N;Yawata, M;LaPointe, VLS;Dickman, MM;Kalmann, R;Imhoff, SM;van Es, JH;López-Iglesias, C;Peters, PJ;Haagmans, BL;Wu, W;Clevers, H;
Cell stem cell
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells, Whole Tissue
Applications: IHC, Western Blot -
Lipocalin 2 activates the NLRP3 inflammasome via LPS?induced NF?kappaB signaling and plays a role as a pro?inflammatory regulator in murine macrophages
Authors: SL Kim, MW Shin, SW Kim
Molecular Medicine Reports, 2022-10-20;26(6):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A timeline of oligodendrocyte death and proliferation following experimental subarachnoid hemorrhage
Authors: Kang Peng, Sravanthi Koduri, Fenghui Ye, Jinting Yang, Richard F. Keep, Guohua Xi et al.
CNS Neuroscience & Therapeutics
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Lipocalin 2 potentially contributes to tumorigenesis from colitis via IL-6/STAT3/NF-kB signaling pathway
Authors: SL Kim, MW Shin, SY Seo, SW Kim
Bioscience Reports, 2022-05-27;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
International Journal of Molecular Sciences, 2022-02-10;23(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
Nature Communications, 2021-12-17;12(1):7333.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model
Authors: AR Schiazza, EG Considine, M Betcher, BD Shepard
Physiological Reports, 2021-12-01;9(23):e15007.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Lipocalin-2 in Inflammatory Breast Cancer Cells with Small Interference RNA and Small Molecule Inhibitors
Authors: GS Santiago-S, R Noriega-Ri, E Hernández-, F Valiyeva, B Quiñones-D, ES Villodre, BG Debeb, A Rosado-Alb, PE Vivas-Mejí
International Journal of Molecular Sciences, 2021-08-10;22(16):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle
Authors: J Brockhaus, RB Craveiro, I Azraq, C Niederau, SK Schröder, R Weiskirche, J Jankowski, M Wolf
Biomolecules, 2021-06-23;11(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exploring the human lacrimal gland using organoids and single-cell sequencing
Authors: M Bannier-Hé, Y Post, J Korving, M Trani Bust, H Gehart, H Begthel, YE Bar-Ephrai, J van der Va, R Kalmann, SM Imhoff, H Clevers
Cell Stem Cell, 2021-03-16;0(0):.
Species: Human, Mouse
Sample Types: Organoid
Applications: IHC -
Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia
Authors: B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha
Cancers (Basel), 2020-07-31;12(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TNF-&alpha controls Lipocalin-2 expression in PC-3 prostate cancer cells
Authors: SK Schröder, A Asimakopou, S Tillmann, S Koschmiede, R Weiskirche
Cytokine, 2020-07-24;135(0):155214.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Renal tubular cell spliced X-box binding protein 1 (Xbp1s) has a unique role in sepsis-induced acute kidney injury and inflammation
Authors: Silvia Ferrè, Yingfeng Deng, Sarah C. Huen, Christopher Y. Lu, Philipp E. Scherer, Peter Igarashi et al.
Kidney International
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Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking
Authors: L Yammine, A Zablocki, W Baron, F Terzi, M Gallazzini
Cell Rep, 2019-11-12;29(7):2067-2077.e6.
Species: Human
Sample Types: Cell Lysate
Applications: IP -
Lipocalin‐2 Protects Against Diet‐Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes
Authors: Yanyong Xu, Yingdong Zhu, Kavita Jadhav, Yuanyuan Li, Huihui Sun, Liya Yin et al.
Hepatology Communications
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CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors
Authors: M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa
Cancer Med, 2019-05-25;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrity
Authors: IT Myszor, Z Parveen, H Ottosson, P Bergman, B Agerberth, R Strömberg, GH Gudmundsso
Sci Rep, 2019-05-08;9(1):7114.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cardiac expression of neutrophil gelatinase-associated lipocalin in a model of cancer cachexia-induced cardiomyopathy
Authors: Vincenzo Musolino, Sandra Palus, Celine Latouche, Micaela Gliozzi, Francesca Bosco, Federica Scarano et al.
ESC Heart Failure
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Innovative in vitro method to study ventilator induced lung injury
Authors: Jon P Joelsson, Iwona T Myszor, Ari J Arason, Saevar Ingthorsson, Paulina Cherek, Gabriel S Windels et al.
ALTEX
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Lipocalin 2 inversely regulates TRAIL sensitivity through p38 MAPK-mediated DR5 regulation in colorectal cancer
Authors: Se-Lim Kim, In Suk Min, Young Ran Park, Soo Teik Lee, Sang-Wook Kim
International Journal of Oncology
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Glycine mitigates renal oxidative stress by suppressing Nox4 expression in rats with streptozotocin-induced diabetes
Authors: Z Wang, J Zhang, L Wang, W Li, L Chen, J Li, D Zhao, H Zhang, X Guo
J. Pharmacol. Sci., 2018-08-25;137(4):387-394.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection
Authors: K Ramani, CV Jawale, AH Verma, BM Coleman, JK Kolls, PS Biswas
JCI Insight, 2018-05-03;3(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cilostazol attenuates kainic acid-induced hippocampal cell death
Authors: YS Park, Z Jin, EA Jeong, CO Yi, JY Lee, IS Park, GS Roh
Korean J. Physiol. Pharmacol., 2017-12-22;22(1):63-70.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells
Authors: YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko
Oncotarget, 2017-11-06;8(62):105536-105552.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares
Authors: Shingo Haneda
J. Reprod. Dev, 2016-12-16;0(0):.
Species: Equine
Sample Types: Luminal Fluid, Tissue Homogenates, Whole Tissue, Whole Tissue
Applications: IHC-Fr, IHC-P, Western Blot -
The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
Sci Rep, 2016-07-07;6(0):29243.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion
Authors: I-Hsiao Chung, Tzu-I Wu, Chia-Jung Liao, Jin-Yo Hu, Yang-Hsiang Lin, Pei-ju Tai et al.
Oncotarget
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Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2
Authors: Khalil El Karoui, Amandine Viau, Olivier Dellis, Alessia Bagattin, Clément Nguyen, William Baron et al.
Nature Communications
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Activation of the unfolded protein response bypasses trastuzumab-mediated inhibition of the PI-3K pathway
Authors: Sreekanth Kumandan, Navin R. Mahadevan, Kevin Chiu, Alexandra DeLaney, Maurizio Zanetti
Cancer Letters
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Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.
Authors: Leung, Lisa, Radulovich, Nikolina, Zhu, Chang-Qi, Organ, Shawna, Bandarchi, Bizhan, Pintilie, Melania, To, Christin, Panchal, Devang, Tsao, Ming Sou
PLoS ONE, 2012-10-04;7(10):e46677.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
Authors: Wortmann A, He Y, Christensen ME, Linn M, Lumley JW, Pollock PM, Waterhouse NJ, Hooper JD
J. Biol. Chem., 2011-10-12;286(49):42303-15.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Lipocalin 2 promotes breast cancer progression.
Authors: Yang J, Bielenberg DR, Rodig SJ, Doiron R, Clifton MC, Kung AL, Strong RK, Zurakowski D, Moses MA
Proc. Natl. Acad. Sci. U.S.A., 2009-02-23;106(10):3913-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
Kidney Int., 2008-10-01;75(3):285-94.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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Recombinant human LCN2 expressed in HEK-293T cells. Test during isolation and purification process.
1st band: 1st eluate (15ug)
2nd band: 2nd eluate (15ug)
3rd band: bead slurry
Dilution: 1:10000
