Human/Mouse/Rat Netrin-1 Antibody

Catalog #: AF6419
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6419
AF6419-SP

Detection of Human, Mouse, and Rat Netrin‑1 by Western Blot.

1 Image

All Netrin-1 Antibodies

Product Details

Citations (6)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Netrin-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Netrin-1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Netrin-1
Val22-Ala604
Accession # O95631

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat Netrin‑1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Netrin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6419) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Netrin-1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-1

Netrin-1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66-76 kDa secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin-1 has multiple receptors, including beta 4-integrins, DCC, DSCAM and UNC5A-thru-D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin-1 is 580 amino acids (aa) in length (aa 25-604). It contains an N-terminal laminin domain (aa 47-284), three EGF-like domains (aa 285-453) and a C-terminal cell-interaction netrin-like domain (aa 472-601). Over aa 22-604, human netrin-1 shares 99% aa sequence identity with mouse netrin-1.

Entrez Gene IDs

9423 (Human); 18208 (Mouse); 396389 (Chicken)

Alternate Names

netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Netrin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells
Authors: G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi
International Journal of Molecular Sciences, 2022-10-22;23(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth
Authors: B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz
Elife, 2020-04-16;9(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus
Authors: CP Prieto, BS Casas, P Falcón, A Villanueva, P Lois, J Lattus, V Palma
Int J Mol Sci, 2019-03-20;20(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC)
Authors: CP Prieto, MC Ortiz, A Villanueva, C Villarroel, SS Edwards, M Elliott, J Lattus, S Aedo, D Meza, P Lois, V Palma
Stem Cell Res Ther, 2017-02-28;8(1):43.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration
Authors: AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma
Oncotarget, 2017-02-07;8(6):9767-9782.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma.
Authors: Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, Klagsbrun M, Smith E
Cancer Res, 2014-05-08;74(14):3716-26.
Species: Human
Sample Types: Whole Cells
Applications: Blocking