Human/Mouse/Rat NM23-H1/H2 Antibody

Catalog #: AF6019 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6019
AF6019-SP

Detection of Human, Mouse, and Rat NM23-H1/H2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat NM23-H1/H2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat NM23‑H1/H2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NM23‑H1
Met1-Glu152
Accession # P15531

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NM23-H1/H2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.2 µg/mL of Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H1/H2 at approximately 17-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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NM23‑H1/H2 in MCF‑7 Human Cell Line. NM23-H1/H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NM23-H1/H2

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17‑20 kDa member of the NDK family of enzymes. NM23‑H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23‑H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2 (Non-metastatic protein 23 homolog 2; also NDKB), and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described.

Long Name

Non-metastatic Protein 23 Homolog 1/Homolog 2

Entrez Gene IDs

4830 (Human)

Alternate Names

AL024257;AWD;GAAD;NB;NBS;NDKA;NDKB;NDPKA;NDPK-A;NDPKB;NDPK-B;NM23;nm23-2;NM23A;NM23B;NM23-H1;NM23-H2;NM23-M1;nm23-M2;NME1;NME2;p18-12d;PUF; NM23-H1/H2; NM23H2