Human/Mouse/Rat PDCD6 Antibody

Catalog #: MAB6015 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6015
MAB6015-SP

Detection of Human, Mouse, and Rat PDCD6 by Western Blot.

1 Image

All PDCD6 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PDCD6 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human PDCD6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) PDCD4 or rhPDCD5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 722232

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PDCD6
Met1-Val189
Accession # O75340

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat PDCD6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDCD6

PDCD6 (Programmed cell death protein 6; also ALG-2 and apoptosis-linked gene 2 protein) is a 22 kDa member of the penta-EF hand protein family of molecules. It is ubiquitously expressed, and performs multiple functions. PDCD6 is contextually linked to apoptosis, proliferation, membrane fusion and vesicle trafficking. Human PDCD6 is 191 amino acids (aa) in length. Although it contains five EF-hand domains, only domains #1 and #3 are considered functional (aa 23-58 and 90‑125). There is one splice form (ALG-2.1) that shows a deletion of Gly121Phe122. This form typically represents about 1/3 of total cellular PDCD6. PDCD6/ALG-2 will homodimerize, and heterodimerize with ALG-2.1. Notably, there are classes of cytosolic proteins that bind PDCD6, but not ALG-2.1, accounting for differences in function. Both human PDCD6 isoforms share 99% aa identity with their mouse counterparts.

Long Name

Programmed Cell Death 6

Entrez Gene IDs

10016 (Human)

Alternate Names

ALG2; ALG-2; Apoptosis-linked gene 2 protein; apoptosis-linked gene-2; FLJ46208; MGC111017; MGC119050; MGC9123; PDCD6; PEF1B; Probable calcium-binding protein ALG-2; programmed cell death 6; programmed cell death protein 6