Home / ERK1/ERK2 / Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

Catalog #: MAB1018
13 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1018
MAB1018-SP
Detection of Mouse, Rat, and Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot.
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Citations (13)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
Source
Monoclonal Rabbit IgG Clone # 269434
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing ERK1 T202/Y204 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse, Rat, and Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) antibody by Western Blot. View Larger

Detection of Mouse, Rat, and Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Rabbit Anti-Human PDGF (Catalog # 120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) in HeLa Human Cell Line. ERK1/ERK2 phosphorylated at T202/Y204 (ERK1) and T185/Y187 (ERK2) was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA using Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Phospho-ERK1/ERK2 antibody in PMA-treated Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-ERK1/ERK2 in PMA-treated Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were unstimulated (light orange open histogram) or treated with 50 ug/mL PMA for 10 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018) or control antibody (Catalog # AB-105-C, blue open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.

Simple Western Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 nM PMA and Ionomycin for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 42 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1/ERK2

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Citations for Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
    Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
    Oncogene, 2022-06-14;0(0):.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Capillary Immunoblot
  2. Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation
    Authors: AM Abu El-Asr, A Ahmad, MI Nawaz, MM Siddiquei, A De Zutter, L Vanbrabant, PW Gikandi, G Opdenakker, S Struyf
    Frontiers in Physiology, 2022-01-10;12(0):807747.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy
    Authors: AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker
    Investigative Ophthalmology & Visual Science, 2021-07-01;62(9):32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Pharmaceutical immunoglobulin G impairs anti-carcinoma activity of oxaliplatin in colon cancer cells
    Authors: Y Shang, X Zhang, L Lu, K Jiang, M Krohn, S Matschos, CS Mullins, B Vollmar, D Zechner, P Gong, M Linnebache
    British Journal of Cancer, 2021-02-09;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Safety and Activity of the Combination of Ceritinib and Dasatinib in Osteosarcoma
    Authors: O Beck, C Paret, A Russo, J Burhenne, M Fresnais, K Steimel, L Seidmann, DC Wagner, N Vewinger, N Lehmann, M Sprang, N Backes, L Roth, MA Neu, A Wingerter, N Henninger, KE Malki, H Otto, F Alt, A Desuki, T Kindler, J Faber
    Cancers (Basel), 2020-03-26;12(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. IGF1R Is a Potential New Therapeutic Target for HGNET-BCOR Brain Tumor Patients
    Authors: N Vewinger, S Huprich, L Seidmann, A Russo, F Alt, H Bender, C Sommer, D Samuel, N Lehmann, N Backes, L Roth, PN Harter, K Filipski, J Faber, C Paret
    Int J Mol Sci, 2019-06-21;20(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
    Authors: X Chang, S Li, XD Xue, F Chang
    Eur Rev Med Pharmacol Sci, 2019-05-01;23(10):4498-4506.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
    Authors: Rachel Barrow-McG
    Nat Commun, 2016-06-23;7(0):11942.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, IHC-Fr, Western Blot
  9. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
    J Immunol, 2013-12-13;192(2):782-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Annexin A10 in human oral cancer: biomarker for tumoral growth via G1/S transition by targeting MAPK signaling pathways.
    Authors: Shimizu T, Kasamatsu A, Yamamoto A, Koike K, Ishige S, Takatori H, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K
    PLoS ONE, 2012-09-17;7(9):e45510.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
    Authors: Lane AE, Tan JT, Hawkins CL
    Biochem. J., 2010-08-15;430(1):161-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells.
    Authors: Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP
    Cancer Lett., 2008-09-07;273(1):127-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody

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Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 10/11/2021
Application: ICC/IF Sample Tested: HeLa cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody MAB1018
Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 06/29/2016
Application: WB Sample Tested: Cell Extracts Species: Human