Home / ERK1/ERK2 / Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

Catalog #: AF1018
41 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1018
AF1018-SP
Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot.
4 Images
Product Details
Citations (41)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing ERK1 T202/Y204 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Multiplex Immunofluorescence
25 µg/mL
Immersion fixed paraffin-embedded sections of human brain cortex

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) antibody by Western Blot. View Larger

Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Phospho-ERK1/ERK2 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # AB-105-C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol.

Immunohistochemistry ERK1/ERK2 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

ERK1/ERK2 in Rat Brain. ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

References
  1. Roskoski Jr., R. (2012) Pharmacol Res. 66:105.
Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice
    Authors: Jinyu Zhang, Ye Lu, Min Jia, Yuying Bai, Lulu Sun, Ziqing Dong et al.
    iScience
  2. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
    Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
    iScience
  3. Crosstalk between protein kinases AKT and ERK1/2 in human lung tumor-derived cell models
    Authors: Aurimas Stulpinas, Matas Sereika, Aida Vitkeviciene, Ausra Imbrasaite, Natalija Krestnikova, Audrone V. Kalvelyte
    Frontiers in Oncology
  4. Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine
    Authors: M Okada, K Fukuyama, E Motomura
    International Journal of Molecular Sciences, 2022-08-14;23(16):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Simple Western
  5. Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems
    Authors: K Fukuyama, E Motomura, M Okada
    International Journal of Molecular Sciences, 2022-06-12;23(12):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Simple Western
  6. High Mobility Group A 1 Expression as a Poor Prognostic Marker Associated with Tumor Invasiveness in Gastric Cancer
    Authors: Hung-Pin Chang, Jen-Tang Sun, Chiao-Yin Cheng, Yao-Jen Liang, Yen-Lin Chen
    Life (Basel)
  7. The potential of organoids in toxicologic pathology: Histopathological and immunohistochemical evaluation of a mouse normal tissue-derived organoid-based carcinogenesis model
    Authors: Rikako Ishigamori, Mie Naruse, Akihiro Hirata, Yoshiaki Maru, Yoshitaka Hippo, Toshio Imai
    Journal of Toxicologic Pathology
  8. O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells
    Authors: Massman LJ, Pereckas M, Zwagerman NT, Olivier-Van Stichelen S.
    Endocrinology
  9. Distinct Effects of Escitalopram and Vortioxetine on Astroglial L-Glutamate Release Associated with Connexin43
    Authors: T Shiroyama, K Fukuyama, M Okada
    International Journal of Molecular Sciences, 2021-09-16;22(18):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Simple Western
  10. Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice
    Authors: Y Machida, T Imai
    Oncology Letters, 2021-08-16;22(4):738.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration and Invasion of Colorectal Cancer through the MAPK Pathway
    Authors: Shuzhen Liu, Guoyan An, Qing Cao, Tong Li, Xinyu Jia, Lei Lei
    International Journal of Molecular Sciences
  12. Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning
    Authors: E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt
    Cells, 2021-01-26;10(2):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages
    Authors: LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang
    Cell Death Differ, 2020-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Age-Dependent and Sleep/Seizure-Induced Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy
    Authors: Fukuyama K, Okada M.
    International Journal of Molecular Sciences
  15. Protective effect of puerarin against burn‑induced heart injury in rats
    Authors: Junling Liu, Jianyun Liu, Mingming Bai, Hui Wang
    Experimental and Therapeutic Medicine
  16. Increased Release of Proinflammatory Proteins in Primary Human Adipocytes and Activation of the Inflammatory NF?B, p38, and ERK Pathways upon Omentin Treatment
    Authors: C Niersmann, K Röhrig, M Blüher, M Roden, C Herder, M Carstensen
    Obes Facts, 2020-04-06;13(2):221-236.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Immunodeficiency in a Patient with 22q11.2 Distal Deletion Syndrome and a p.Ala7dup Variant in the MAPK1 Gene
    Authors: Ana I. Sánchez, Mary A. García-Acero, Angela Paredes, Rossi Quero, Rita I. Ortega, Jorge A. Rojas et al.
    Molecular Syndromology
  18. Fluid supplementation accelerates epithelial repair during chemical colitis
    Authors: JF Burgueño, JK Lang, AM Santander, I Fernández, E Fernández, J Zaias, MT Abreu
    PLoS ONE, 2019-04-19;14(4):e0215387.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. mTOR-dependent phosphorylation controls TFEB nuclear export
    Authors: Gennaro Napolitano, Alessandra Esposito, Heejun Choi, Maria Matarese, Valerio Benedetti, Chiara Di Malta et al.
    Nature Communications
  20. Characterization of TRKA signaling in acute myeloid leukemia
    Authors: SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M
    Oncotarget, 2018-07-10;9(53):30092-30105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation
    Authors: Z Wan, C Xu, X Chen, H Xie, Z Li, J Wang, X Ji, H Chen, Q Ji, S Shaheen, Y Xu, F Wang, Z Tang, JS Zheng, W Chen, J Lou, W Liu
    J. Cell Biol., 2018-04-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  22. Ferric citrate and ferric EDTA but not ferrous sulfate drive amphiregulin-mediated activation of the MAP kinase ERK in gut epithelial cancer cells
    Authors: NM Scheers, DIA Pereira, N Faria, JJ Powell
    Oncotarget, 2018-03-30;9(24):17066-17077.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Glycans modify mesenchymal stem cell differentiation to impact the function of resulting osteoblasts
    Authors: KM Wilson, AM Jagger, M Walker, E Seinkmane, JM Fox, R Kröger, P Genever, D Ungar
    J. Cell. Sci., 2018-02-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice
    Authors: BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli
    Front Cell Neurosci, 2017-10-09;11(0):313.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. In vitro and in vivo anti-tumor activity of alectinib in tumor cells with NCOA4-RET
    Authors: Sachiko Arai, Kenji Kita, Azusa Tanimoto, Shinji Takeuchi, Koji Fukuda, Hiroshi Sato et al.
    Oncotarget
  26. Use of non-selective ?-blockers is associated with decreased tumor proliferative indices in early stage breast cancer
    Authors: A Montoya, CN Amaya, A Belmont, N Diab, R Trevino, G Villanueva, S Rains, LA Sanchez, N Badri, S Otoukesh, A Khammanivo, D Liss, ST Baca, RJ Aguilera, EB Dickerson, A Torabi, AK Dwivedi, A Abbas, K Chambers, BA Bryan, Z Nahleh
    Oncotarget, 2017-01-24;8(4):6446-6460.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
    J Biol Chem, 2016-02-24;291(19):10277-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014-01-28;9(1):e86466.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors.
    Authors: Nanjo, Shigeki, Yamada, Tadaaki, Nishihara, Hiroshi, Takeuchi, Shinji, Sano, Takako, Nakagawa, Takayuki, Ishikawa, Daisuke, Zhao, Lu, Ebi, Hiromich, Yasumoto, Kazuo, Matsumoto, Kunio, Yano, Seiji
    PLoS ONE, 2013-12-26;8(12):e84700.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Role of NADPH oxidase isoforms NOX1, NOX2 and NOX4 in myocardial ischemia/reperfusion injury
    Authors: Vincent Braunersreuther, Fabrizio Montecucco, Mohamed Asrih, Mohammed Ashri, Graziano Pelli, Katia Galan et al.
    Journal of Molecular and Cellular Cardiology
  31. Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells.
    Authors: Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T
    Anticancer Res, 2013-04-01;33(4):1547-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways.
    Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G
    PLoS ONE, 2012-09-17;7(9):e45257.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011-04-11;17(11):3619-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer.
    Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S
    Clin. Cancer Res., 2011-01-10;17(8):2260-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. PKCalpha-induced drug resistance in pancreatic cancer cells is associated with transforming growth factor-beta1
    Authors: Ying Chen, Guanzhen Yu, Danghui Yu, Minghua Zhu
    Journal of Experimental & Clinical Cancer Research
  36. Deoxygedunin, a natural product with potent neurotrophic activity in mice.
    Authors: Jang SW, Liu X, Chan CB
    PLoS ONE, 2010-07-13;5(7):e11528.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells.
    Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD
    Biochem. Biophys. Res. Commun., 2009-08-04;388(1):51-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion.
    Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S
    J. Mol. Cell. Cardiol., 2009-01-07;46(5):612-20.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  39. E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
    Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
    Int. J. Cancer, 2008-10-15;123(8):1741-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
    Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
    Mol. Ther., 2007-05-15;15(8):1531-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Autocrine role of angiopoietins during megakaryocytic differentiation.
    Authors: Saulle Ernestina, Guerriero Raffaella, Petronelli Alessia et al.
    PloS One

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Western Blot Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody AF1018
Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Punitha Senthil on 01/12/2018
Application: WB Sample Tested: PANC-1 human pancreatic carcinoma cell line Species: Human
Western Blot Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody AF1018
Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Jothi Jayavel on 01/11/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Western Blot Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody AF1018