Human/Mouse/Rat Phospho-PTEN (S380) Antibody

Catalog #: AF2838
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2838
AF2838-SP

Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-PTEN (S380) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PTEN when phosphorylated at S380 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human PTEN S380 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot. Western blot shows Human/Mouse/Rat PTEN Monoclonal Antibody (Catalog # MAB847) immunopreciptate of MRC-5 human embryonic lung fibroblast cell line, mouse and rat brain tissue untreated (-) or treated (+) with 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/ Rat Phospho-PTEN (S380) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2838), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PTEN (S380) at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: PTEN

The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

References

Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Entrez Gene IDs

5728 (Human); 19211 (Mouse); 50557 (Rat)

Alternate Names

BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product is covered under US Pat. Nos. 6,262,242 and 6,482,795 and other pending patent applications.

Related Research Areas

Citations for Human/Mouse/Rat Phospho-PTEN (S380) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Blockade of Axl signaling ameliorates HPV16E6-mediated tumorigenecity of cervical cancer
Authors: EH Lee, KY Ji, EM Kim, SM Kim, HW Song, HR Choi, BY Chung, HJ Choi, HW Bai, HS Kang
Sci Rep, 2017-07-18;7(1):5759.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expression.
Authors: Kechagioglou, Petros, Papi, Rigini M, Provatopoulou, Xeni, Kalogera, Eleni, Papadimitriou, Elli, Grigoropoulos, Petros, Nonni, Aphrodit, Zografos, George, Kyriakidis, Dimitrio, Gounaris, Antonia
Anticancer Res, 2014-03-01;34(3):1387-400.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot