Human/Mouse/Rat PP2C epsilon /PPM1L Antibody

Catalog #: MAB6914 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6914
MAB6914-SP

Detection of Human, Mouse, and Rat PP2C epsilon /PPM1L by Western Blot.

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Human/Mouse/Rat PP2C epsilon /PPM1L Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.

Source

Monoclonal Mouse IgG_2B Clone # 700612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PP2C epsilon /PPPM1L
Asp43-Arg178
Accession # Q5SGD2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat PP2C epsilon /PPM1L by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C e/PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C epsilon/PPM1L

PP2C epsilon, also known as PPM1L, is a 34 kDa (predicted) widely expressed Ser/Thr phosphatase. It consists of a 25 amino acid (aa) extracellular domain, a 17 aa transmembrane segment, and a 318 aa cytoplasmic region (aa 43-360) which contains the phosphatase domain (aa 91-344). Alternate splicing generates additional isoforms of human PP2C epsilon with 179 aa or 127 aa N-terminal truncations or a 166 aa C-terminal truncation. The PP2C epsilon -mediated dephosphorylation of TAK1 and ASK1 is regulated by oxidative stress and inflammatory cytokines. Loss of PP2C epsilon function is associated with the development of metabolic syndrome. Within aa 43-178, human PP2C epsilon shares 99% aa sequence identity with mouse and rat PP2C epsilon.

Long Name

Protein Phosphatase 2C alpha

Entrez Gene IDs

151742 (Human); 242083 (Mouse); 310506 (Rat)

Alternate Names

EC 3.1.3; EC 3.1.3.16; MGC132545; MGC132547; PP2C epsilon; PP2CE; PP2CEProtein phosphatase 2C isoform epsilon; PP2C-epsilon; PPM1L; PPM1-LIKE; protein phosphatase 1 (formerly 2C)-like; protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C epsilon isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1L